Search results for "HIC" in Articles / App Notes
Article
Tackling Analytical Method Development for ADCs
Their composition--a biologic linked to a small-molecule cytotoxic, which enables targeted delivery--also creates challenges for analytical method development, both for characterization and lot releas…
Article
Evolution of the Monoclonal Antibody Purification Platform
Hydrophobic-interaction chromatography (HIC) in bind and elute mode has been used to achieve further clearance of product-related impurities.
• Virus filtration step is the method of choice as…
Article
Top challenges in recombinant protein purification process development
Well, for recombinant proteins, you often don't have an affinity solution, an affinity chromatography solution that can be used, which is very different from mAbs, which do have a great affinity solut…
Article
Enhancing Resins Addresses Purification Concerns
The mRNA vaccines in particular are still rather new to the market, which is why no such platform processes seem to be in place.”
One common feature of all mRNA-based APIs is the poly(A) tai…
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Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Several years ago, we started a development project to design and validate the next-generation protein A resin, MabSelect PrismA, which was just introduced to the market. Now, 1M NaOH can in fact be u…
Article
Protein Purification Troubleshooting Guide
Includes general tips and specific help for SEC, AC, IEX, and HIC*. Get practical advice on small changes and active choices that can significantly impact your purification results.
Article
Optimizing KiH bispecific antibody chromatography steps
… step for two closely related knob-into-hole (KiH) bsAbs using MabSelect PrismA™ protein A resin which reached a purity of 94%. In addition, two flow-through polishing steps using orthogonal binding …
Article
Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
The first method is using nonaffinity chromatography steps (AIEX + heat treatment + HIC), while the second method is using the novel affinity Cytiva™ Protein Select™ resin for the capture step, follow…
Article
Chromatography for the Diversified Antibody Pipeline
… Helding: The advantage of producing monoclonal antibodies is that we can apply platform processes which makes the development and manufacturing easy. As we shift to a diverse pipeline this may be di…
Article
Fundamentals of Hydrophobic Interaction Chromatography
This article explores the fundamentals and applications of hydrophobic interaction chromatography using Capto™ resins to increase productivity.
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