Search results for " cell-culture" in Articles / App Notes
Article
Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
However, adhesion degree is not the only factor to be considered when choosing the most convenient cell detaching method within a given cell-culture process. The authors consider different factors and…
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
The cell-culture process and media are known to have a significant effect on the expression and stability of therapeutic products as well as their critical quality attributes (CQAs) including glycosyl…
Article
Implications of Cell Culture Conditions on Protein Glycosylation
Richard Easton
This article reviews the implications of cell-culture conditions on biologic product quality, focusing on glycosylation and analytical techniques for its accurate assessment. Glyco…
Article
Ensuring Viral Safety of Viral Vaccines and Vectors
PCVs entered vaccine processes via porcine trypsin, a common cell-culture reagent. In 2014, using next-generation sequencing (NGS), FDA’s Center for Biologics Evaluation and Research (CBER) retrovirus…
Article
An Integrated Approach to Ensure the Viral Safety of Biotherapeutics
Although several serum-free and chemically defined nutrient media formulations are available for commercial use, many cell-culture applications require use of nutrient media supplementation with serum…
Article
Use of Multivariate Data Analysis in Bioprocessing
MVDA applications in cell-culture operations
Cell-culture operations are by far the most common platform used for production of protein therapeutics. In this endeavor, various advanced offline and…
Article
Novel Vaccine Technologies Meet the Need for Pandemic and Therapeutic Solutions
Examples include cell-culture, synthetic DNA, chimeric antigen, and recombinant protein nanoparticle vaccine technologies.
Egg-vaccine technology limitations
Traditional vaccine manufactu…
Article
Challenges in Cell Harvesting Prompt Enhancements
Cell harvesting involves the separation of the cell-culture from the medium and running it through several techniques: centrifugation, microfiltration, depth filtration, and filtration through absolut…
Article
Single-Use Technologies Prove Effective for Viral Vector Process Development
They are also designed to increase cell-culture density while maintaining adequate gas exchange and supply of nutrients and preventing accumulation of cell culture byproducts, according to Tan.
Fo…
Article
Mapping a Route for Cell and Gene Therapy Process Development
In this context, he identifies multiple important challenges:
- Modification of 2D cell-culture systems into a scalable, three-dimensional (3D) suspension-culturesystems with adequate control s…