Search results for "Recovery"
Article
Optimizing capture and polishing steps in an rAAV purification process
The chromatography purification described includes affinity capture to maximize recovery with efficient impurity removal.
READ FULL ARTICLE >>
Article
Enhancing Resins Addresses Purification Concerns
… chromatography will gain even further market share based on its ability to achieve high purity and recovery in just one step,” Ihre says. Another possible game changer, he notes, would be if a unive…
Article
Maximum Output Starts with Optimized Upstream Processing
…nes, using technologies to achieve high cell densities and perfusion cultures, and optimizing virus recovery. In addition, Lipinski notes that the use of high-efficacy viral vector platforms to minim…
Article
Cell-free Expression Systems Pose Cell Culture Alternative
That complexity ranges from the creation of a master cell bank all the way to manufacturing at scale, product recovery and purification, and, finally, product formulation, according to a group comment…
Article
Developing a Closed, Connected Single-Use mAb Purification Process
In the Protein A step, Fibro PrismA is compared with MabSelect PrismA regarding process time, recovery, and purification performance.
Article
Updating Viral Clearance for New Biologic Modalities
Viral clearance processes and guidance must evolve along with newer biotherapeutic modalities.
By Feliza Mirasol
Ensuring viral clearance (i.e., removal) in downstream purification has becom…
Article
Eliminating Residual Impurities Starts with a Strategic Plan
If derivatization is necessary to improve the sensitivity and detectability of the analyte in question, any loss in recovery must be accounted for. Filtration of samples must not cause any interferenc…
Article
Flexible Facilities for Viral Vector Manufacturing
Single-use and modular systems will meet demand for rapid implementation at different scales.
By Jennifer Markarian
Industry experts have noted that there are limitations in the…
Article
The Evolving Role of Starting Materials in Cell and Gene Therapy
Post-thaw recovery of MSCs in culture for a 24-hour period allows these cells to fully regain their functional potency (10).
Cell therapy source material losses resulting from sub-optimal cell p…
Article
Addressing the Complex Nature of Downstream Processing with QbD
Pressure excursions, including what may happen during product recovery buffer flushes, have sometimes been shown to lead to viral break-through during validation studies. We have adopted a risk mitiga…