Search results for " media"
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Platform Approach Speeds Process Development
Chinese hamster ovary (CHO)-based cell culture platforms should combine state-of the-art technologies for genetic vectors, host cell portfolio, cell line generation, cultivation media, and process des…
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Ensuring the Biological Integrity of Raw Materials
Stopping contamination
The most common type of contamination incident happens when a media component is contaminated. For example, bovine serum can be contaminated with reovirus, epizootic hemorrh…
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Tools for Continuous Bioprocessing Development
The device can use up to 324 cultures simultaneously, allowing it to be used for process optimization, screening, and media optimization (14, 15).
• Most recently, Sartorius Stedim’s TAP ambr 15 an…
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Use of Multivariate Data Analysis in Bioprocessing
Similarly, application of surface-enhanced Raman scattering (SERS) spectroscopy for simple and fast analysis of cell-culture media degradation has been demonstrated (16). In this work, chemometric too…
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QbD and PAT in Upstream and Downstream Processing
Timing of events and generation of basal and feed media are controlled with traditional process controls and standard operating procedures. Online cell-density measurement is an area of active develop…
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Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The typical platform process is generally comprised of an initial capture step with Protein A-chromatography, a first polishing step with cation-exchange media, and a final polishing step using anion-…
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Re-use of Protein A Resin: Fouling and Economics
The cost of Protein A resin is nearly 50% higher than traditional chromatographic media with nonproteinaceous ligands (2, 10). Resin cost for a large column (>1-m diameter) can exceed $1 million. What…
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Cell-Culture Advances Test Bioreactor Performance Models
The continual development of higher-producing cell lines, the switch to chemically defined media, and other advances in cell-culture technology are, however, challenging developers of modeling and sim…
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Unifying Continuous Biomanufacturing Operations
John Bonham-Carter, director of upstream sales at Repligen, says Repligen estimates the median run length for new processes in clinical trials today is approximately four weeks, with approximately fou…
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Implications of Cell Culture Conditions on Protein Glycosylation
These differences in glycosylation can have significant effects on the quality of the therapeutic protein produced, as can the choice of cell clone, the basal and feed media used, and the cell-culture…