Search results for " concentration" in Articles / App Notes
Article
Getting a Handle on Biopharma’s Most Critical Quality Attributes and Quality Control
Upstream, the most important CQAs include glucose concentration and cell mobility; downstream, they include turbidity, pH, and conductivity. In addition, virus, microbe, and endotoxin monitoring and c…
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Mechanistic Modeling of Preparative Ion-Exchange Chromatography
For example, for the commonly used ion-exchange chromatography, pH, ion concentration, gradient, and stationary phase can affect the recovery step as well as the resulting product quality.
For com…
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Optimization of Protein Expression in Escherichia Coli
However, this is problematic with the “all-or-nothing” induction characteristics of the T7-based systems, because low inducer concentration leads to a mixed population. This population is typically co…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Some improvements have been made to decrease the development time of IEC, such as the development of a quaternary solvent system to simultaneously adjust the pH and salt concentration to reduce buffer…
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Implementing Inline Conditioning to Advance Process Intensification
Buffer preparation is still a highly manual activity that often requires a lot of resources due to the large number and overall volume of buffers. With inline conditioning buffers can be prep…
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Improving Upstream Predictability
The company is using in-line sensors and Raman spectroscopy to enable precise measurement of viable cell concentration and individual cell metabolites such as glucose and lactate, he explains. Using r…
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Moving PAT from Concept to Reality
…es (size-exclusion chromatography and ultra-high-performance liquid chromatography [UHPLC]); product concentration (HPLC); and protein charge variants (ion exchange chromatography). Numerous at-line …
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What’s New in Upstream Technologies
Bench-scale automated cell culture system
The scale-X carbo, from Univercells, is a bench-scale automated cell culture system for the expression and concentration of viral drug substance suited fo…
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NIST Spectroscopic Measurement Standards
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Ultraviolet (UV) absorbance measurements provide a rapid and reliable method to determine protein concentrations. The National Institute of Standards and Technology (NIST) has developed Standard R…
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Process Chromatography: Continuous Optimization
Process development scientists also need to consider the ability to achieve good capacity and separation under higher salt concentrations for added flexibility and process simplicity, according to Par…