Search results for "chromatography"
Poster
Fast Trak Services: a collaborative project to accelerate downstream biosimilar process development
Process optimization focused on improving the affinity chromatography (AF) capture step as well as the intermediate purification and polishing steps, using hydrophobic interaction chromatography (HIC…
Article
Fusion Proteins Pose Manufacturability Challenges
“These fusion proteins include an Fc region, are taken from an IgG, that extends the serum half-life of the fusion partner, facilitates expression, and allows purification by protein A affinity chroma…
Article
Accelerated flavivirus vaccine production with modern tools and solutions
Single-use production bioreactors and chromatography purification columnsmitigate cross-contamination risk and support increased operator safety, while reducing time to market by eliminating costly an…
Article
Evaluating E&L Studies for Single-Use Systems
Analytical methods
In the three case studies, the following analytics were applied:
• Headspace sampling/gas chromatography/mass spectrometry (HS/GC/MS) and gas chromatography/mass…
Article
A Platform Approach to Purification of Antibody Fragments
However, with recent developments of novel affinity chromatography resins, there are new emerging possibilities. Here, we present affinity chromatography resins that offer the possibility of a platfor…
Article
Single-use technologies in downstream process intensification
When using conventional chromatography solutions, cleaning and cleaning validation consumes a considerable amount of time and facility resources. Cleaning-in-place protocols are performed before, duri…
Poster
Residual DNA analysis in influenza vaccine processing
In cell-based influenza vaccine production, the European Pharmacopoeia demands a host cell residual DNA concentration of less than 10 ng per dose. To reliably measure residual DNA in both process sa…
Poster
Process economy effects of modernizations in vaccine purification
One technique is core bead chromatography, which allows for dual functionality, combining size separation with bind-elue chromatography. This poster describes the technique using influenza virus produ…
Article
Automated harvesting and 2-step purification of unclarified mammalian cell-culture broths containing antibodies
We report on a new integrated platform for automated harvesting of whole unclarified cell-culture broths, followed by in-line tandem affinity-capture, pH neutralization and size-exclusion chromatograp…
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
MS is further used in combination with high-performance liquid chromatography (HPLC) and other chromatographic methods to detect and localize amino acid modifications (13). Chromatography techniques a…