Search results for "chromatography"
Poster
Purification platform challenges and strategies for handling of "tricky" MAbs
In the work presented here, capture using high-capacity protein A medium (resin) was followed by polishing using a novel cation exchange chromatography medium.
Article
Biopharma Advances Demand Specialized Expertise
Ultra performance liquid chromatography (UPLC) systems have improved resolution and sensitivity, while reducing run times. In addition, 2D high-performance liquid chromatography (HPLC)/high-resolution…
Article
A Bright Future for the Plasma Fractionation Industry
Improvements in more established process operations, such as filter press operation and chromatography processes, were also reported.
Among the quality and regulatory trends, Quality-by-Design (…
Article
Detecting Protein Aggregates and Evaluating their Immunogenicity
This technique helps link liquid chromatography or capillary electrophoresis electrospray (CESI) easily and allows orthogonal techniques to work together for better information content. With peptide m…
Article
Securing the Single-Use Supply Chain
…re once its facility was in place, sourcing only critical disposable components such as filters and chromatography sorbents. Although disposable systems do provide numerous advantages, such as reduce…
Article
Mechanistic Modeling: Does it Have a Future in Process Development?
For some unit operations, such as ion-exchange chromatography, mechanistic models are easily employed for prediction. However, when it comes to reverse-phase chromatography or HIC, the mathematical de…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
Antibody titer was measured using high-performance liquid chromatography (HPLC) (Waters Corporation) equipped with a protein A column (Applied Biosystems). The protein charge profile was analyzed by i…
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Predicting Progress in Protein Aggregation
Some examples include modified immunoassays, self-interaction chromatography, and visual viscosity determination based on the diffusion of latex beads. “Unfortunately, there have been very few such te…
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Elucidating Biosimilars Characterization
Using liquid chromatography-mass spectrometry (LC-MS) methods, we can determine and confirm the primary sequence, the presence and identity of post-translational modifications and perform protein stru…
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Automated Concentration and Diafiltration of Multiple siRNA Samples
Following a manual deprotection of the siRNA product, reversed-phase high-performance liquid chromatrography (RP-HPLC) and liquid chromatography–mass spectrometry (LC/MS) are used to analyze the quali…