Search results for "chromatography"
Article
Selecting a Comprehensive Bioburden Reduction Plan
Chromatography resins, for example, are not typically provided sterile or capable of being sterilized, so they are a potential source of contaminations unless cleaned, sanitized, and stored correctly.…
Article
Host-Cell Protein Measurement and Control
Other technologies, such as 2D-differential in-gel electrophoresis (DIGE), capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) or two-dimensional-liquid chr…
Article
Advanced Technologies Facilitate Scale-up and Technology Transfer
He adds that better-designed model bioreactor, chromatography, and filtration systems are allowing for even smoother scale-up and tech-transfer operations.
Raw material choices are also being adju…
Poster
Optimizing dynamic binding capacity and aggregate clearance in an mAb polishing step
Capto™ S ImpAct is a strong cation exchange (CIEX) chromatography medium (resin) designed for monoclonal antibody (MAb) polishing steps. In this study, optimization of binding conditions targeting h…
Article
Optimization of Protein Expression in Escherichia Coli
For example, MBP will bind to amylose-agarose, while GST will bind to glutathione-agarose for purification by affinity chromatography. Similar to affinity tags, these fusion partners must be cleaved o…
Article
USP Publishes Monoclonal Antibody Guidelines
The material is an IgG1, 2mg/vial lyophilized presentation, which is used in the size-exclusion chromatography assay (SEC) and the capillary sodium dodecyl sulfate (SDS) electrophoresis (reduced and n…
Article
Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
Quantification of TBPP, oxidized TBPP, and bDtBPP was performed with ultrahigh-performance liquid chromatography (UHPLC) with Exactive Orbitrap high-resolution accurate mass mass spectrometry (HRAM MS…
Poster
Accurate comparability assessment of a biosimilar interferon in process development
This application note describes how to achieve accurate comparability assessments using Biacore™ T200 and
Amersham™ WB system in the development of a biosimilar. This is exemplified in this study…
Article
DoE Provides Benefits, but Preparation Is Necessary
It is also important to reduce the data to single-value results as often as possible, such as the percent monomer or aggregate as determined using size exclusion chromatography-high performance liquid…
Article
A Q&A With Phil Lester, co-organizer of the HTPD Conference
For the Olympiad, Cytiva created a kit containing a target protein in a crude feedstock along with a variety of chromatography media and suggested analytical methods. The goal is to purify the target …