Search results for " process" in Articles / App Notes
Article
Optimization of Protein Expression in Escherichia Coli
Depending on the purpose of protein expression, these tags may be left in the final product or cleaved off with the use of specific proteases during the purification process.
Proteases such as Tev …
Article
Optimizing Resin Performance with Disposable Chromatography Solutions
They are designed to improve downstream processing performance, ensure consistency of product, reduce cost, and reduce time between batch runs. Total suite, labor, and material costs decrease dramatic…
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Q&A with Industry Leaders: Rita Peters
One example is Cytiva’s acquisition of the HyClone line cell culture media, sera, and process liquids; Dharmacon RNAi gene modulation, products; and Sera-Mag magnetic beads. With the changes in compan…
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Predicting Progress in Protein Aggregation
As the development of biologic drugs based on antibodies and other protein/peptide molecules continues apace, the control and prevention of aggregation during manufacturing, downstream processing, for…
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Advancing QbD in the EU
In 2003, EMA set up a team to back activities in process analytical technology (PAT) in the EU. The agency has seen its PAT team as a key element in the implementation of QbD by encouraging process un…
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Quality Considerations for Using AI in Bio/Pharma
But does the regulated nature of bio/pharmaceutical development, manufacturing, and commercialization serve up unique challenges for incorporating AI into processes? According to Siegfried Schmitt, vi…
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Reviewing the Importance of Biosimilarity and Interchangeability
Identifying and quantifying impurities should include process-related impurities, degradation products, and contaminants. This is a crucial aspect of biosimilar development, Stamm notes. “Analytical m…
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Introducing Supor Prime Filters: A Filtration Solution for High-Concentration Therapies
It is harder to process fluid through the filter and it clogs faster. Manufacturers are compensating for this with oversized filters that have more hold-up. In a highly concentrated drug, every drop c…
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Extractables in Single-Use Systems Used in ADC Manufacturing, Part 2
The low levels of extractables found in this study support the use of ReadyToProcess™ columns, ÄKTA readyflux™ flow kits, and ÄKTA ready™ flow kits in ADC processes.
Read the application …
Article
Alternative solutions to separate AAV full and empty capsids using anion exchange
Key for a successful process are high overall yields of full capsids with effective empty capsid reduction and efficient impurity removal.
Here we present high-performance anion exchange (AEX) chr…