Search results for " glycosylation"
Article
Platform Technologies Improve Protein Expression
Tools enabling modification of metabolic pathways for glycosylation in algae and yeast glycosylation are also noteworthy. “These approaches offer avenues for designing better fit-for-purpose molecules…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
It has now been adapted to permit the evaluation of candidate cell lines expressing Fab, Fc fusion proteins, proteins (with or without glycosylation), as well as bispecific or bifunctional molecules. …
Article
Tools for Continuous Bioprocessing Development
The FDA ruling stated that the glycosylation of the products manufactured at each scale differed, and, thus, the 2000-liter product required a new biologic license application (1).
Why is process …
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Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
Figure 1: Changes of critical quality attributes, such as high-order structure, amino acid modifications, glycosylation, and stability might impact binding activity and concentration. All figures are …
Article
Continuous Manufacturing: A Changing Processing Paradigm
Apr 01, 2015
By Randi Hernandez
BioPharm International
Volume 28, Issue 4
Although continuous manufacturing is well established for bulk chemicals, complex automation and validation …
Article
Optimizing your Cell Culture Process to Improve Productivity
We also offer protein quality analysis, including charge variance, glycosylation, and protein aggregation. These types of results provide the scientists information on critical attributes of the prote…
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Virus-like Particles as Therapeutic Moieties of the Future
PTMs such as glycosylation can also be analyzed using lectin-based glycan differentiation assay (16). Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) can be used to analyze the m…
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Moving PAT from Concept to Reality
“Trace metals, glucose, lactate, pH, DO, amino acid content, glycosylation profiles, and HCPs must be measured to monitor the process and enable feedback/feedforward control to optimize yield (in-line…
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Modeling the Degradation of mAb Therapeutics
Modeling the Degradation of mAb Therapeutics
Kinetic models can be used to study aggregation and fragmentation to help ensure stability.
By Anurag S. Rathore, Rohit Bansal
…
Article
Process Chromatography: Continuous Optimization
That requires removal of closely related byproducts in proteins, such as charge or glycosylation variants,” Bulpin says. He adds that the drive away from bind/elute chromatography steps to more effici…