Search results for " buffer"
Article
Trends in Downstream Bioprocessing
• Buffer capacity, column size, UF membrane area are all problems.
• Primary clarification is a major bottleneck.
• We need multiple trains.
• We need better affinity columns (protein A). …
Article
The Development and Application of a Monoclonal Antibody Purification Platform
After anion exchange, the flow-through fraction containing the antibody was passed through a nanofiltration unit to remove potential viruses and then formulated in buffer in a final concentration and …
Article
Automated Concentration and Diafiltration of Multiple siRNA Samples
The collection sequence was programmed as shown below:
Tube 1: Reservoir volume at product end
Tube 2: Product chase
Tube 3: Reservoir volume at buffer flush end
Tube…
Article
Evaluating the Use of Continuous Chromatography
When used with a perfusion or fed-batch bioreactor, continuous chromatography can help reduce the amount of resins and buffers needed. It also provides greater flexibility for processes and uses a sma…
Article
Top Process Development Trends for 2022 and a Look into 2023
Also, this interview shares insights related to moving from manual buffer preparation to implementing inline conditioning for automated buffer management.
A Smarter, More Digital Future
Invest…
Article
Using Digital Twins to Model Process Chromatography
[These conditions] may include a changing feedstream quality with respect to product or contaminant concentrations, variations in material quality, such as adsorber capacity and buffer composition, or…
Article
What If You Could Remove Your Antibody Aggregates Already in the Protein A Step?
A design of experiments approach was used to study the influence of four factors on the removal of HMW species: (1) elution buffer type, (2) conductivity of the elution buffer, (3) pH gradient length …
Article
Process Development: 2020 Reflections and 2021 Possibilities
mAb manufacturing productivity can be greatly improved by optimizing the buffer and media preparation using innovative approaches that cut back on the time and effort associated with these components.…
Article
Scaling Up Novel Therapies
Prime examples, he says, are recipes with long and shallow linear gradients, as well as pH specifications for process buffers that can only be achieved off-line in the lab. Developers may also use a w…
Article
Reducing Cross-Contamination Risks in Process Chromatography
Often disposable buffer and collection bags are used across all scales. “Chromatography is a case study for the combined application of single-use and reusable components,” he says.
A continuo…