Search results for " buffer" in Articles / App Notes
Article
A Q&A on Bioprocessing Trends and Technologies at INTERPHEX 2016
Did you see any trends in the consumables segment?
The quality and supply of cell-culture media, buffers, and other process liquids was also a popular topic. Demand for custom formulations and pac…
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
Another practical approach for media identification is the use of relatively simple tests for the identification of process buffers (14). In such case, few nonspecific identification tests—such as com…
Article
Continuous Manufacturing: A Changing Processing Paradigm
As the mass of protein to be purified increases, required buffer and elution volumes in batch-capture chromatography increase (8). A continuous capture option could be a good alternative to reduce col…
Article
Trends in Downstream Bioprocessing
• Buffer capacity, column size, UF membrane area are all problems.
• Primary clarification is a major bottleneck.
• We need multiple trains.
• We need better affinity columns (protein A). …
Article
The Development and Application of a Monoclonal Antibody Purification Platform
After anion exchange, the flow-through fraction containing the antibody was passed through a nanofiltration unit to remove potential viruses and then formulated in buffer in a final concentration and …
Article
Automated Concentration and Diafiltration of Multiple siRNA Samples
The collection sequence was programmed as shown below:
Tube 1: Reservoir volume at product end
Tube 2: Product chase
Tube 3: Reservoir volume at buffer flush end
Tube…
Article
Evaluating the Use of Continuous Chromatography
When used with a perfusion or fed-batch bioreactor, continuous chromatography can help reduce the amount of resins and buffers needed. It also provides greater flexibility for processes and uses a sma…
Article
Top Process Development Trends for 2022 and a Look into 2023
Also, this interview shares insights related to moving from manual buffer preparation to implementing inline conditioning for automated buffer management.
A Smarter, More Digital Future
Invest…
Article
Using Digital Twins to Model Process Chromatography
[These conditions] may include a changing feedstream quality with respect to product or contaminant concentrations, variations in material quality, such as adsorber capacity and buffer composition, or…
Article
What If You Could Remove Your Antibody Aggregates Already in the Protein A Step?
A design of experiments approach was used to study the influence of four factors on the removal of HMW species: (1) elution buffer type, (2) conductivity of the elution buffer, (3) pH gradient length …