Search results for " Transfection"
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rAAV Production Using HEK293 Cell Line and Transfection Medium
Improving the performance of a scalable AAV process in Xcellerex™ XDR-10 and XDR-200 bioreactor using HyClone™ peak expression medium.
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Commercial Production of Gene Therapies Using Suspension or Adherent Cell Lines
This is, most often done using transient transfection as a means of integrating the viral genome into the cells, although stable producer cells lines aredeveloped and used (3). Mammalians cells, espec…
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Single-Use Technologies Prove Effective for Viral Vector Process Development
The presence of serum, culture harvest viability, turbidity, cell lysis reagents, contaminant DNA levels, and residual transfection agents are all upstream factors. Overall process scalability and rob…
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New Therapies Present Scaling Challenges
One major challenge to a scale-up strategy lies in the crucial step of transient transfection, which is when DNA is introduced into the mammalian cell. The genetic material to be introduced into the m…
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A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
…tion of transcript integrity of those transgenes expressed transiently and stably in CHO cells after transfection but prior to single-cell cloning of the candidate production cell lines using single-…
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Best Practices for Selecting a Top-Quality Cell Line
“Equally important,” stresses Fisch, “is the quality of the recombinant DNA expression and transfection systems. Incorporating recombinant DNA stably into a cell line’s genome in a manner that promote…
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Gene Therapies Push Viral Vector Production
Moreover, some of the cells are sensitive to chemical transfection reagents. Furthermore, because a cell-harvesting process involves several steps, the probability of committing mistakes is much highe…
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Cell culture process development for AAV vector production in suspension cells
Adaptation of HEK293 suspension cell line for transient transfection and scalable adeno-associated virus (AAV) production.
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Platform Approach Speeds Process Development
All steps during CLD, including transfection, selection, and cultivation in suspension, are currently carried out in chemically defined serum-free media. Multiple CHO host cell lines with different gl…
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Advances in Sterilization Technologies for Overcoming Viral-Vector Manufacturing Challenges
Sterility must be assured for all biologic drug products. Terminal sterilization achieved via treatment with heat, radiation, or certain chemicals (vaporous hydrogen peroxide, vaporous peracet…