Search results for "filtration"
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
Protein recovery was ≥ 99%, after 5‑times concentration and 5‑fold diafiltration. The process was performed according to set specifications for a tangential flow filtration (TFF) process, with the…
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Best Practices in Qualification of Single-Use Systems
Single-use buffer filtration system and storage bag application is common in downstream processes. Final drug-product sterile filtration and transfer using SUS has also being increasingly applied to f…
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Using Single-Use Technologies in Downstream Processing
Some examples are filtration systems, buffer preparation systems, and sampling/dispensing systems. Key advantages of using single-use systems are speed of implementation, which is crucial for high-imp…
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Trends in Downstream Bioprocessing
It involves the removal of insoluble cell debris and particulates; product isolation through extraction, adsorption, ultrafiltration, or precipitation; product purification through affinity chromatogr…
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The Development and Application of a Monoclonal Antibody Purification Platform
After harvest, the cells were removed from the cell culture broth by depth filtration. In some cases, the broth was concentrated before the first chromatography step because of facility fit issues. Pr…
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Automated Concentration and Diafiltration of Multiple siRNA Samples
Manual crossflow filtration (CFF) steps of the process lacked process robustness and were a source of variation between production runs. The project objective was to streamline the process to improve …
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Extractables in Single-Use Systems Used in ADC Manufacturing, Part 2
We performed extractables studies on single-use chromatography and tangential flow filtration systems using common solvents in ADC manufacturing, DMA and DMSO. The low levels of extractables f…
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Design Considerations for a Commercial Cell and Gene Therapy Facility
For mAb bioprocessing and viral-vector manufacturing, the equipment needed is similar, such as single-use bioreactors and standard harvest and downstream systems for clarification, tangential flow fil…
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Single-Use for Downstream Chromatography: Benefit or Hindrance?
“Clarification via depth filtration or tangential flow membrane filtration (TFF) is just one example.”
As this trend continues, the concept of an end-to-end single-use manufacturing facility has mo…
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Perfusion Culture Using TFF or ATF as Cell Retention Method
The work demonstrates perfusion culture of an antibody producing Chinese hamster ovary (CHO) cell line, using either tangential flow filtration (TFF) or alternating tangential flow filtration (ATF) as…