Search results for "filtration"
Article
Connected Polishing and Concentration Under One Automation Method
Here, the ÄKTA™ ready chromatography system was directly connected to the ÄKTA readyflux filtration system, for polishing and concentration of a target molecule. One automation method was cr…
Article
Being Thorough When Transferring Technology
…p from lab scale to commercial scale in biologics?
Moscariello and Young (CMC Biologics): Depth filtration and centrifugation are not always scalable for manufacturing operations. However, having…
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Efficient concentration of a low-titer bovine IgG with high recovery in low volume
The use of ReadyCircuit™ single-use assemblies allowed for aseptic connectivity of the filtration device. Filtration was performed using ReadyToProcess™ hollow fiber filter cartridges. The described …
Article
Continuous Processing for the Production of Biopharmaceuticals
…er separation technologies such as continuous chromatography, refolding, precipitation, cell lysis, filtration, and aqueous two-phase extraction (3). Table I presents a summary of recent efforts in t…
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Considerations in depth filter scale-up
Depth filters are widely used in the biopharmaceutical industry. They are typically made of a mixture of cellulose, filter aids, and a binder. These components can be milled and mixed in diffe…
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Instructional Videos for Bioprocess Equipment
Cytiva offers free on-demand instructional videos that will help you with operating bioreactors, chromatography and filtration systems, including flow kit installation on single-use systems. Modules a…
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Digitalization: The Route to Biopharma 4.0
For formulation, a model for predicting volumetric concentration factor across single pass tangential flow ultrafiltration (SPTFF) can be used to develop real-time automated monitoring and a control s…
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High-concentration monoclonal antibody drugs: manufacturing challenges
Manufacturing unit operations that are directly impacted by higher concentration formulations range from ultrafiltration to sterile filtration, filling, mixing and storage. Solutions that maintain qua…
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Technology Innovations Improve Process Chromatography Performance
…rated with other product flow-through purification steps, such as anion exchange polishing or virus filtration, without requiring conductivity adjustment or dilution.”
While some bioprocessing is…
Article
Eliminating Residual Impurities Starts with a Strategic Plan
The selected isolation method—precipitation, filtration, centrifugation, extraction, etc.—should not affect the impurities with respect to concentration or composition. If derivatization is necessary …