Search results for " dAb"
Poster
Scale-up and process economy calculations of a dAb purification process using ready-to-use products
A three-step dAb purification process was used as model. The results show that although operating expenses can be more extensive when scale and number of batches increase, ready-to-use equipment and r…
Article
E. coli growth and Dab expression in single-use and stainless steel fermentors
Cell growth,
cell density, and Dab expression levels were assessed and compared between the two production vessels. The results show that the performance of the XDR-50 MO fermentor is comparable …
Article
A Platform Approach for the Purification of Domain Antibodies (Dabs)
This platform approach, based on a capture step with Capto L, enables increased efficiency and productivity in developing therapeutics based on Dabs.
Click here to download the application not…
Article
Exploring the world of antibodies: mAbs, Fabs, dAbs, and bsAbs
There is a vast array of antibody variants, including multispecific (msAb), bispecific antibodies (bsAb), antibody fragments (Fab, dAb, scFv), and antibody-drug conjugates (ADC). But with this…
Article
Testa Challenge 2020 Attracts Leading International Bioprocessing Innovators
The 2020 challenge required entrants to show how their product or service would improve a real-life start to finish bioprocess run in the Testa facility by producing and purifying a dAb domain antibod…
Article
High-Throughput Process Development in an Historical Environment
The challenge was to use HTPD workflows for maximized Dab purity and yield.
A workshop held by Ashley Hesslein from Bayer HealthCare kicked off the meeting. The topic was “Lessons Learned in Build…
Article
Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
Protein L resins bind to kappa light chain and are often used to purify antibody fragments such as dAbs and Fabs — but they can also be used to remove product-related impurities from bispecif…
Article
Protein L affinity resin is ready to move to manufacturing
After comparing resin performance for mAb, Fab, and dAb samples, MabSelect™ VL protein L resin offered increased binding capacities versus other resins. This improvement could address existing protein…
Article
Antibody Production in Microbial Hosts
Figure 1: Structure of an antibody and its fragments: A) Antibody (IgG class); B) antibody fragments (VH, scFv, Fab, scFab, dAb, scFv-CH3). Abbreviations are: variable heavy chain (VH), variable light…
Article
Chromatography processes for the diversified therapeutic antibody pipeline
The diversity of antibody variants in today’s pipeline presents unique challenges for purification and we see molecules such as bsAbs, Fabs, dAbs, and others. Conventional chromatography protocols, …