Search results for " two-step"
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Optimization of a two-step purification method for bispecific antibodies
A two-step purification protocol was further developed to increase the process economy.
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A scalable single-use two-step plasmid purification process
The current global demand for biopharmaceuticals is over USD 300 billion, with an estimated growth of ≥ 12% annually. While MAb processes have become much more efficient, production of mRN…
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Optimization of a Two-Step Purification Method for Bispecific Antibodies
Alligator Bioscience AB develops antibody-based pharmaceuticals for cancer treatment, specializing in mono- and bispecific antibodies for tumor-directed immunotherapies. Alligator and Cytiva devel…
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Exotoxin Clearance from mAb Samples in a Two-Step Chromatography Process
A heat-stable exotoxin can remain as a contaminant of a drug substance throughout the whole manufacturing process, with the risk of adversely affecting patients’ health. One such toxin is cereulide …
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Efficient purification of meningococcal polysaccharides in a two-step chromatography workflow
This application note demonstrates the purification of meningococcal capsular polysaccharides using chromatography. The described process constitutes a viable alternative to the traditional proce…
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Evolution of the Monoclonal Antibody Purification Platform
These results led to inclusion of a two-step elution strategy where a high pH (4-5) wash followed by low pH (3-4) elution was used to minimize the fragment content in the Protein A product pool. When …
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Modern plasmid purification process
AcuraBio selects Cytiva's latest two-step purification protocol to offer a productive plasmid DNA service to its customers globally to alleviate supply constraints for mRNA and cell & gene th…
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Queue Up Your Chromatography Steps
To save time and increase throughput, many scientists consider increasing the level of automation in their protein purification workflows. Learn how you can set up automated multi-step protocols uti…
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Process Chromatography: Continuous Optimization
Biosimilars and other challenges
The increased trend toward the development of two-step chromatography processes and higher protein doses is requiring more performance from chromatography products, …
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Comparing Protein A Resins for Monoclonal Antibody Purification
This was followed by a two-step washing procedure to remove unbound particles. Bound antibody was eluted with 50 mM acetic acid and the column resin was cleaned in place with five column volumes (CV) …