Search results for " purification" in Articles / App Notes
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Increasing upstream titers can make the Protein A chromatography process step the rate-limiting step in downstream purification. Protein A chromatography columns are frequently too large, limiting the…
Article
Improving Process-Scale Chromatography
By Cynthia Challener, PhD
Chromatography is a crucial step in the purification of biopharmaceuticals and accounts for a significant portion of downstream processing efforts (time and cost) for bio…
Article
Downstream Processing Continues to Worry CMOs
…hese problems, respondents were asked to identify actions they had implemented to improve downstream purification operations.
Among all survey respondents, the most common action taken was to c…
Article
Viral Clearance Challenges in Bioprocessing
42-44
Removal and inactivation of adventitious and endogenous viruses have traditionally been achieved during the downstream purification of biologics. Viral clearance is assessed in small-scale te…
Article
Evaluating the Use of Continuous Chromatography
In downstream processing, continuous chromatography is gaining traction in the purification of protein therapeutics. As the biopharmaceutical industry strives to demonstrate, continuous manufacturing …
Article
Preclinical Evaluation of Product Related Impurities and Variants
The level of these species, however, is usually controllable through fermentation and purification steps. There is no consensus among the regulatory agencies on the maximum allowable limit for these s…
Article
Process Chromatography Selection for Downstream Processing Applications
Important factors such as column design, resin development, and automation have all played a role in simplifying the purification process, in some cases reducing it from five steps to three (1). Selec…
Article
Optimizing Resin Performance with Disposable Chromatography Solutions
The chromatography medium is the means by which protein purification is performed, and it must perform as expected, otherwise the purity of the drug can be impaired. Customers have expressed that they…
Article
Bioprocessing Advances in Vaccine Manufacture
…portable bioprocessing facilities
•Novel expression systems/improved cell lines
•New purification technologies.
As these technologies advance and are increasingly adopted for…
Article
Exosome isolation by tangential flow filtration and size exclusion chromatography
We describe an evaluation of purification workflows from three different cell culture supernatants. The workflows we describe are suitable for scale-up and combine tangential flow filtration (TFF) for…