Search results for " purification" in Articles / App Notes
Article
Insights from process developers - top challenges in recombinant protein purification
You might be one of the many process developers who are working on a diversity of molecules such as enzymes, peptides, scaffolds, and other recombinant proteins. This constantly evolving molecul…
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eBook: Purification of Antibody Therapeutics - Approach for Diversified Pipeline
Antibody therapeutics are the largest class of biotherapeutics. Over the years, development beyond traditional monoclonal antibodies (mAbs) has increased. Today, we see multispecific and bispe…
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Optimization of a HIC step for recombinant protein purification
Follow this step-by-step high-throughput process development (HTPD) workflow to determine the optimal resin and initial conditions for hydrophobic interaction chromatography (HIC).
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Evaluation of Fibro PrismA for Scalable Single-Use mAb Purification
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In clinical monoclonal antibody (mAb) manufacturing, capture on protein A chromatography resin gives excellent recovery and purity. However, because clinical batch siz…
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A Closer Look at Affinity Ligands
Breaking down affinity ligands
BioPharm: What are affinity ligands and how do they contribute to the downstream purification process?
Bekker (BioCina): Affinity ligands are molecules that can bi…
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A Look at the Affinity Chromatography Landscape
With the advent of next-generation biopharmaceuticals, chromatography material suppliers have been challenged to develop affinity ligands and support systems that enable the separation and purificatio…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
The standardization of cellular expression systems, bioreactor conditions, purification processes, manufacturing hardware, and disposables has resulted in faster clinical development and lower costs. …
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Process Chromatography: Continuous Optimization
“Different ion exchange chemistries can offer unique selectivity depending on the purification needs, so a variety of chemistries are tested based on the application,” Parra says.
Affinity resins …
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Trends in Downstream Bioprocessing
It involves the removal of insoluble cell debris and particulates; product isolation through extraction, adsorption, ultrafiltration, or precipitation; product purification through affinity chromatogr…
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Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
Downstream Processing and Purity Analysis
For downstream laboratory-scale purification, performed using standard, non-disposable equipment, approximately 10 L virus harvest from the upstream proce…