Search results for " resins"
Article
Development of Purification for Challenging Fc-Fusion Proteins
In this study, both CEX and HIC resins were evaluated in a flow-through mode as a polishing step in purification of Fc-fusion proteins that previously proved challenging to purify using conventional A…
Article
Process Chromatography Selection for Downstream Processing Applications
For these molecules, chromatographic techniques such as ion-exchange, hydrophobic interaction chromatography, and multimodal resins are common. Affinity resins like Capto L (antigen-binding fragments …
Article
SEC in the Modern Downstream Purification Process
Kevin O'Donnell
BioPharm International
Volume 3, Issue 28
Since the introduction of commercial chromatography resins approximately six decades ago, size-exclusion chromatography (SEC) ha…
Article
Evolution of the Monoclonal Antibody Purification Platform
Though extremely efficient and effective, the step primarily suffers from the significantly high cost of Protein A resins in comparison to other popular modes of chromatography (such as ion exchange).…
Article
Top Tips for Successful Development of Antibody Chromatography Processes
Jeanne Linke Northrop: So what are your suggestions in regards to addressing the challenges?
Jakob Liderfelt: I would say it's built on understanding the different resins and having a deeper…
Article
Technology Innovations Improve Process Chromatography Performance
It is of great importance to rely on materials, such as resins, buffers and equipment, which are designed for production purposes and available in the amount they are needed,” he states.
“The othe…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
The HTS method has also been applied to the development of other purification processes for unique biotherapeutic modalities where screening of a large number of resins may be required.
Another cons…
Article
Improvements in Protein A Chromatography
However since 1978, the capacity and productivity of Protein A resins have improved at a rate of about 5% a year. The net effect of this improvement is that newer resins are an order of magnitude bett…
Article
Addressing the Complex Nature of Downstream Processing with QbD
“For instance, protein A or affinity resins in general, as well as flow through steps, display these properties since these steps in most cases build on a fundamental understanding of the physiochemic…
Article
The Challenge of Disruptive Technologies in Bioprocessing
“The Thermo Scientific POROS CaptureSelect technology provides highly selective affinity resins with antibody-like specificity, which can significantly improve the biomolecule purification process, ma…