Search results for " fed-batch" in Articles / App Notes
Article
Optimization of Fed‑Batch Culture Conditions for a mAb‑Producing CHO Cell Line
The selected Cell Boost supplements were thereafter applied in fed-batch cultures to fine-tune their relative ratios. The selected fed-batch culture conditions supported mAb titers of approximately 4 …
Article
Continuous Manufacturing: A Changing Processing Paradigm
…ge portfolio of short-run manufacturing products, and “scaling process development is easier using fed-batch technologies” (1). Langer also writes that, based on data from his yearly report, “there h…
Article
Evaluating the Use of Continuous Chromatography
…usly, which is a useful approach when the purification step is connected to an upstream perfusion or fed-batch bioreactor, and, by having two or more columns in the loading zone, you can overload the…
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Achieving Process Balance with Perfusion Bioreactors
As with batch and fed-batch processes, single-use bioreactors offer numerous other advantages over stainless steel. “Users can eliminate most of the cleaning and validation steps that are associated w…
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Trends in Single-use Bioreactors
Another example of how single-use systems can facilitate continuous processing is the ease with which a single-use bioreactor that has been operated in a fed-batch manufacturing process can be readily…
Article
Digitalization: The Route to Biopharma 4.0
Glycosylation Flux Analysis Reveals Dynamic Changes of Intracellular Glycosylation Flux Distribution in Chinese Hamster Ovary Fed-Batch Cultures. Metab. Eng. 2017, 43, 9–20. DOI: 10.1016/j.ymben.2017.…
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Biomanufacturing: Demand for Continuous Bioprocessing Increasing
Negative assessments from within the industry of continuous perfusion fed-batch processing overall may reflect a lack of direct exposure or experience with continuous technology.
In BioPlan’s a…
Article
Maximizing the Effectiveness of Upstream Bioprocessing Through the Combination of Lab Informatics and Instrumentation
…roductivity with their WuXiUP continuous perfusion-based processing platform compared to traditional fed-batch, which enables 1000-L single-use bioreactors to produce the same amount as 10,000-L stai…
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Cell-Culture Advances Test Bioreactor Performance Models
He points to the higher cell densities in perfusion mode, which result in increased oxygen demand in a higher viscosity solution with potentially more cell-free biopolymers and lipids than a fed-batch…
Article
Scaling Up Novel Therapies
One way they do this, he says, is by “scaling out,” intensifying processes, for example by using fed-batch and perfusion on the upstream side, and multicolumn operations, whether it’s just higher cycl…