Search results for " fed-batch" in Articles / App Notes
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
CHO cells in fed-batch mode at low glutamine (17% (w/w) hampered the rate of deamidation up to 40%. Polyols at high concentrations displayed a protective effect on oxidation. Addition of 0.01% (v/v) P…
Article
Innovations in Perfusion Bioprocessing
In this study, we present a method for deriving perfusion culture media by leveraging existing fed-batch media and feeds.
Furthermore, we illustrate the successful transfer of the develop…
Article
A Small-Scale Model to Increase Yield in Cell Culture Perfusion of mAb at Large Scale
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As process intensification is adopted into large-scale manufacturing, the responsibility on scale-down models to accurately represent the expanded operating space quickly follow…
Article
Explore the Process Intensifier App
Explore alternatives to intensify your processes >>
How do you know if an intensification approach will give you advantages over your current biomanufacturing process, and how do you choose the …
Article
The Importance of Process Intensification and PAT for Achieving Real-Time Release
As the industry has matured, effective manufacturing platforms and analytical tools and technologies appropriate for fed-batch manufacturing in stainless-steel or single-use bioreactors have also adva…
Article
Developing a Continuous Large-Scale Perfusion Cell Culture Process
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Perfusion processes enable continuous operation over extended periods of time by constantly providing fresh nutrients for the cells and simultaneously removing spent…
Article
Developing a Continuous Large-Scale Prefusion Cell Culture Process
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Perfusion processes enable continuous operation over extended periods of time by constantly providing fresh nutrients for the cells and simultaneously removing …
Article
Improving Upstream Predictability
“We’ve consistently been seeing titers in the range of 4–6 g/L in [monoclonal antibody] mAb fed-batch processing within a chemically defined media environment. This environment, in turn, results in mo…
Article
Best Practices for Selecting a Top-Quality Cell Line
Irina Shi - Stock.Adobe.com
Leveraging automation and a step-by-step approach are keys to success.
By Cynthia A. Challener
Cell lines determine the performanc…
Article
Moving PAT from Concept to Reality
Intensified processes are much more complex than conventional fed-batch processes and therefore require tighter monitoring and control, adds Grieb. “PAT and automation do not only provide this, but al…