Search results for " binding"
Article
Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
In this study, we compared two approaches for purifying a recombinant protein that does not have an affinity binding partner.
The first method is using nonaffinity chromatography steps (AI…
Article
Get Started: Purification of mAb Variants - eLearning
These molecules show wider variation in binding affinities and are often more challenging to separate from impurities, such as host cell proteins (HCP), host DNA, aggregates, and isoforms.
In this …
Article
Chromatography innovations for the diversified antibody pipeline
Our MabSelect™ VH3 affinity resin interacts with the variable heavy chain of the VH3 sequence family of human antibody, delivering high binding capacity and efficient separation of product-related imp…
Webcast
Cytiva Protein Select Technology Live Webinar
The protocol does not require the use of a protease, and the same buffer can be used for equilibration, binding, wash, and elution.
Article
Video: Standardize recombinant protein purification process development
This technology standardizes purification during process development of any protein that does not have an affinity binding partner.
WATCH THIS VIDEO TO LEARN MORE
Article
Report from the 6th International HTPD Conference
Calculated descriptors for proteins and resins could then provide predictions of product binding and optimal screening regions for separation. This QSAR models were followed by the mechanistic modelin…
Article
Balancing AAVs Versus LLVs
Knowledge of adeno-associated variant's infection pathway is based on only a few serotypes. The prevailing understanding is that serotypes bind to different, or have differential affiniti…
Article
Get more from UNICORN™ software with plug-in extensions
Access extensions for UNICORN software from the new Cytiva Marketplace, including a free extension that performs Dynamic Binding Capacity (DBC) calculations for breakthrough volume and QB valu…
Article
Optimization of a two-step purification method for bispecific antibodies
Bispecific antibodies (bsAbs) have gained increasing interest over recent years because they can bind two antigens, offering unique modes of action compared to monoclonal antibodies. New and i…
Article
Download your Mechanistic Modeling eBook
It also uses adsorption isotherms to quantify how molecules compete for ligands when binding.
Why use mechanistic modeling in downstream process development?
With mechanistic models, proc…