Search results for " purity" in Articles / App Notes
Article
Multidimensional Scale-Up of a Monoclonal Antibody Capture Step, Using ÄKTA™ pilot 600
Equivalent mAb recovery and purity was achieved between the larger and the smaller scales, showing a robust and scalable process.
Click here to view the application note.
Article
Development of Purification for Challenging Fc-Fusion Proteins
Process capacity determination with column chromatography
The screening above with the desired purity was used as a starting condition for small-scale column experiments at a higher load …
Article
Process Chromatography: Continuous Optimization
Improved resin chemistries and customized separation solutions are enabling more efficient separations.
By Cynthia A. Challener
Cell-culture and fermentation are high-…
Article
From Darwin to Recombinant Fc Multimers
…t, together with a QbD approach, delivered a robust process control strategy to deliver a high-level purity and quality recombinant VIIa (rhFVIIa) from a somewhat unusual expression system: milk of t…
Article
Managing Residual Impurities During Downstream Processing
Increased understanding of potential impurities has spurred efforts to standardize monitoring procedures.
By Cynthia A. Challener
Manufacture of protein and other biologic drug substances via …
Article
Improvements in Protein A Chromatography
Alternative technologies like CEX capture or precipitation often provide lower purity and require more mAb-specific development.
What future directions would you like to see in the development o…
Article
Critical Quality Attributes Challenge Biologics Development
While CQAs of small-molecule drugs can affect the purity, strength, drug release, and stability of a drug, the complex nature of biologics can create additional quality attributes that can affect the …
Article
Mechanistic Modeling of Preparative Ion-Exchange Chromatography
This specificity is of particular relevance to the production of biotech therapeutics, which require high standards of purity. However, development and optimization of a process chromatography step pr…
Article
Accelerated flavivirus vaccine production with modern tools and solutions
This white paper includes a Fast Trak Services case study on the use of such resins to increase purity and yield of a flavivirus vaccine manufacturing process.
Download white paper.
Article
Simplify Every Step of Histidine-Tagged Protein Purification
A purity between 60% and 80% was achieved when using a manual purification workflow. To improve this, an automated purification approach was evaluated. Learn more in this blog post how automated his…