Search results for " column"
Article
Speed and Flexibility Are Dual Goals for Biopharma Operations
Other product introductions included new chromatography columns, inline process monitoring sensors, lab and pilot-scale filtration systems, and pumps and fluid handling systems. Suppliers also introdu…
Article
The Tide Stays High
There have even been suggestions that a venture capitalist might be appointed to run the agency (no appointments had been announced at the time this column was written). However, emerging bio/pharma i…
Article
Safety Drives Innovation in Animal-Component-Free Cell-Culture Media Technology
Resins, columns, tubing, connectors, filters, gaskets, mixing blades, and other process contact materials or ingredients are included in this scope. The producers of these items also need to understan…
Article
Single-use technologies in downstream process intensification
In bioprocessing, thorough cleaning is critical in order to take full benefit of the resin by removing impurities. When using conventional chromatography solutions, cleaning and cleaning validation …
Poster
Residual DNA analysis in influenza vaccine processing
There are many commercially available DNA preparation kits that use different techniques to bind DNA, from spin columns with a DNA-binding membrane or resin to magnetic beads. However, these kits are …
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
Folic acid method
The folic acid method utilized an ultra-high performance liquid chromatography (UHPLC) system from Waters Acquity equipped with photodiode array detector (PDA) and Acquity UHPLC …
Article
Antibody Fragments and Their Purification by Protein L Affinity Chromatography
In the case of recombinant full-length antibodies with suitable Fc regions, two or three column purification processes centered around Protein A affinity chromatography have proven to be fast, efficie…
Article
Best Practices in Qualification of Single-Use Systems
The author discusses the current best practices in technical qualification of single-use systems.
By Weibing Ding, PhD
Single-use technology has been around for approximately two decades if …
Article
Handbook: Size Exclusion Chromatography Principles and Methods
The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns.
Download now.
Article
Detecting Protein Aggregates and Evaluating their Immunogenicity
“This method provides absolute determination of molar mass, independently of SEC column calibration, so we can be certain of the identification of eluting species.” Larger, irreversible aggregates of …