Search results for " column" in Articles / App Notes
Article
Unifying Continuous Biomanufacturing Operations
Downstream continuous purification has seen some success as well, mostly through the integration of various chromatography column sequences. But the continuous production of biologics in a closed, tot…
Article
Continuous Manufacturing: A Changing Processing Paradigm
They also note that continuous capture typically requires sterile chromatography columns. “For downstream polishing steps, the infancy of multi-column chromatography systems is a limitation,” they add…
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
… molecular standards is not effective unless the proteins under study have similar conformations and column interactions as those of the standards. The combination of SEC with downstream light scatte…
Article
Evaluating the Use of Continuous Chromatography
…ation step is connected to an upstream perfusion or fed-batch bioreactor, and, by having two or more columns in the loading zone, you can overload the first column [because] any product/target molecu…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
…and elution conditions, can be explored in a shorter time frame compared with when using small-scale columns, which are operated in a sequential mode. In this study, a robotic liquid handling system …
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
The method performs very well despite changes to column temperature, protein load, and buffer pH. The data from these robustness experiments reflect the expected range for the system suitability resul…
Article
Reducing Cross-Contamination Risks in Process Chromatography
Preparatory and post-processing steps such as column packing, validation of packed-bed efficiency, verification of endotoxin clearance, unpacking, cleaning of unpacked columns, etc., can also be elimi…
Article
Improving Process-Scale Chromatography
Capacity in a capture step such as Protein A is critical in reducing cost and increasing the ease of use through reductions in column size, buffer usage, and overall footprint of the unit operation,” …
Article
Downstream Processing Continues to Worry CMOs
(Figure courtesy of author)
Chromatography and ultrafiltration
When it comes to specific steps that contribute to downstream-related capacity constraints, chromatography columns continue to be…
Article
Using Quality by Design to Develop Robust Chromatographic Methods
The column screening strategy employed in our laboratories and for the examples discussed here encompasses four stationary phases; two organic solvents; and acidic, neutral, and basic aqueous mobile p…