Search results for " column" in Articles / App Notes
Article
New Selection Guide: Ion Exchange Chromatography Columns and Resins
Use these decision trees to find the most appropriate resin for your needs.
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Article
Optimizing Resin Performance with Disposable Chromatography Solutions
The experts addressed how disposable chromatography columns can be used throughout the life of a campaign, the costs and benefits associated with using prepacked columns, the complexities of resin str…
Article
Process Chromatography Selection for Downstream Processing Applications
Important factors such as column design, resin development, and automation have all played a role in simplifying the purification process, in some cases reducing it from five steps to three (1). Selec…
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Making the Move to Continuous Chromatography
Switching from batch to continuous allows for improved resin capacity utilization through continuous loading (overloading, in fact) of the column or membrane for increased process efficiency. It is pa…
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Re-use of Protein A Resin: Fouling and Economics
A typical Protein A chromatography process consists of five steps: pre-equilibration and equilibration of the column; capture of the mAbs from the harvest; wash step with the selective buffer to remov…
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SEC in the Modern Downstream Purification Process
The hydrodynamic properties of the molecules in the sample may be determined, for example, by comparing the chromatogram of the sample to be purified on a high-performance liquid chromatography column…
Article
Mechanistic Modeling of Preparative Ion-Exchange Chromatography
Mass-transfer models
The transport of solute through the various phases inside the packed bed column has been described using mathematical models, albeit with different level of details (1). Mecha…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
The HMW1 flowed through the column and the peak contained primarily dimer species (HMW2). The CHA step had a capacity of 20 g/L.
The HTS analyses also indicated that a HIC resin under certain loa…
Article
Continuous Chromatography: Trends, Definitions and Approaches
All of the acronyms describe the same thing: a chromatography capture step-based utilizing multiple columns operated in a merry-go-round manner, where columns moved against the direction of feed flow.…
Article
Development of Purification for Challenging Fc-Fusion Proteins
In this study, utilizing a combination of high-throughput condition screening in 96-well plates, optimization in small-scale column models, and a novel virus-spiking strategy, the authors have develop…