Search results for " column" in Articles / App Notes
Article
Modeling and Miniaturization: Key Topics at Successful HTPD Conference
Several talks focused on the utilization of miniaturized columns, such as Robocolumns, and if they could become an even more important tool going forward and especially in late -tage development. For …
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Protein A chromatography columns are frequently too large, limiting the use of prepacked devices and consuming larger than necessary quantities of buffer.
Cytiva stated in a recent press rel…
Article
Process Chromatography: Continuous Optimization
As suppliers expand their portfolios of resins to enable efficient separations of newer biomolecules, they must also consider the potential to increase the column capacity, binding/ separation capacit…
Article
Improvements in Protein A Chromatography
At the same time we see promise in non-bead based protein A technologies like membrane adsorbers and monoliths, which have higher productivity than packed columns due to their short bed heights and hi…
Article
Cost Considerations Drive Lean Technology in Biopharmaceutical Manufacturing
Traditional manufacturing processes for biologics make use of large, fed-batch reactors and oversized chromatography columns that end up wasting time or material. Alternatively, upstream process scale…
Article
Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
Approximately 350 µg protein was loaded onto the column. The detection wavelength was 280 nm. The column equilibration (65 minutes) and sample elution (35 minutes) was done with the mobile phase (50 m…
Article
Technical and Process Economical Aspects of Using Capto™ Q and ReadyToProcess™Adsorber Q in mAb Polishing
Compared with conventional chromatography, using stainless steel columns, the use of ReadyToProcess Adsorber membranes can help reduce capital investment, consumption of buffers and other process liqu…
Article
Use of Multivariate Data Analysis in Bioprocessing
In yet another application, chemometrics has been applied to predict column integrity and impurity clearance during reuse of the chromatographic resin (24). The authors in that study presented a metho…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
The percent monomer is determined by size exclusion high performance liquid chromatography, using two G3000 SWXL columns (Tosoh, Montgomeryville, PA) in series with detection of the antibody at 214 nm…
Article
Extractables in Single-Use Systems Used in ADC Manufacturing, Part 2
The low levels of extractables found in this study support the use of ReadyToProcess™ columns, ÄKTA readyflux™ flow kits, and ÄKTA ready™ flow kits in ADC processes.
Read the application …