Search results for " affinity"
Article
Next Generation Process Chromatography
Binding capacities, range of selectivity, and the stability of affinity ligands have been vastly improved. One of the main remaining challenges is that each purification cycle today typically takes >3…
Article
Preclinical Evaluation of Product Related Impurities and Variants
First, the binding affinity of GCSF variant to GCSF-R followed the order: Reduced> Pure=Oxidized=f-Met> Aggregate. The enhanced binding affinity of the reduced GCSF samples was attributed to stabiliza…
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Antibody Production in Microbial Hosts
Recent developments in aglycosylated antibodies possessing similar antigen-binding affinity and engineered-specific FcγR binding has led to an enormous interest in these products. In this article, the…
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Re-use of Protein A Resin: Fouling and Economics
Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography has remained the de facto capture step for purification of monoclonal antibod…
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Challenges of Protein Aggregation During Purification
For example, certain CaptureSelect affinity ligands/resins from Thermo Fisher Scientific have been designed to favor elution under less acidic, more neutral pH conditions, according to Gebski.
Ref…
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Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
The first method is using nonaffinity chromatography steps (AIEX + heat treatment + HIC), while the second method is using the novel affinity Cytiva™ Protein Select™ resin for the capture step, follow…
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Chromatography innovations for the diversified antibody pipeline
Our MabSelect™ VH3 affinity resin interacts with the variable heavy chain of the VH3 sequence family of human antibody, delivering high binding capacity and efficient separation of product-related imp…
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Dynamic Binding Capacity in Fiber-Based Chromatography
Fiber chromatography matrices such as Fibro PrismA can bind a large amount of protein in a fraction of the purification time required for an affinity resin. Just like with resins, the dynamic binding …
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Selection Guide: Columns and Resins for Antibody Purification and Immunoprecipitation
Download the latest version of the selection guide Columns and Resins for Antibody Purification and Immunoprecipitation to help you select the most suitable affinity column for purifying your antibody…
Article
Process Chromatography Selection for Downstream Processing Applications
By contrast, immobilized metal affinity chromatography is not often used because tagged proteins are uncommon in process scale.
Becerra-Arteaga (MilliporeSigma): Ion-exchange chromatography is mos…