Search results for " affinity"
Article
New Therapies Present Scaling Challenges
New Therapies Present Scaling Challenges
Manufacturing differences between traditional mAb therapies and newer biotherapeutics dictate whether processes should be scaled up, scaled out, or use an al…
Article
Single-Use for Downstream Chromatography: Benefit or Hindrance?
For example, one unmet user need is the lack of affinity and ionexchange chromatography beads that are sufficiently cost-effective to be classified as single-use. These process steps continue to be th…
Article
Biopharmaceutical Process Development: 2018 Lessons and 2019 Predictions
…ey to More Effective Vaccines
• Evaluation of Performance of a Disposable mAb Affinity Chromatography Column Used Over Multiple Process Cycles
• Optimization of Fed‑Batch Culture Condit…
Article
Scaling Up Novel Therapies
Scaling Up Novel Therapies
Platform processes have improved monoclonal antibody scale-up. Can they do the same for personalized therapies?
By Agnes S…
Article
Ensuring Viral Safety of Viral Vaccines and Vectors
Chromatography has been shown to remove PCV (33), and ion exchange and affinity chromatography have been shown to purify vectors such as AAVs. However, because achieving robust clearance is challengin…
Article
Cleaning Protein A Resins with 1 M NaOH
With increasing mAb titers, the impurity level of the cell culture feed increases. If not removed, these impurities can build up on the protein A resin and start to leach into the process material i…
Article
Chromatography Scale-Up: Don’t Get Tied Down by Bed Height
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When scaling, you can make better use of existing chromatography columns while avoiding overcapacity.
Keeping a constant residence time is a well-est…
Article
Capacity and Performance of a Next Generation Protein A Chromatography Resin
MabSelect™ PrismA is a protein A resin of which both the ligand and the base matrix have been optimized. The resin exhibits improved capacity over its predecessor MabSelect SuRe™ products, while maint…
Article
Making the Move to Continuous Chromatography
He points to some affinity processes that need to have extended contact times and need to run slowly; the efficiencies of continuous chromatography would not be realized in these cases. Switching from…
Article
Your Protein Purification Questions Discussed
In one of Cytiva´s web chats, ÄKTA™ system users asked questions relating to lab-scale protein purification and ÄKTA systems, which experts answered during a live session.
The expert…