Search results for " affinity" in Articles / App Notes
Article
From Darwin to Recombinant Fc Multimers
Damien Bataille from LFB presented how affinity chromatography with a novel chromatography resin, VIISelect, together with a QbD approach, delivered a robust process control strategy to deliver a high…
Article
Fusion Proteins Pose Manufacturability Challenges
“These fusion proteins include an Fc region, are taken from an IgG, that extends the serum half-life of the fusion partner, facilitates expression, and allows purification by protein A affinity chroma…
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Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
Enormous success achieved with mAbs can be attributed to their distinctive beneficial properties and advantages, which include high binding specificity and affinity, availability of humanized forms th…
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Educational Protein Purification Videos
You can shape tomorrow’s researchers today by providing them with skills essential for modern protein research laboratories. Here you will find useful resources to support you in teaching protein pu…
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Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
…nt analysis) or by comparing and even fitting entire binding curves for determination of kinetic and affinity parameters. SPR systems can deliver a direct binding assay focused on relevant interactio…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
…cells, and the initial mAb capture step was carried out with an alkali-stabilized, Protein A-derived affinity medium using a standard protocol. An HTPD approach was then taken for the subsequent puri…
Article
A Bright Future for the Plasma Fractionation Industry
Expanded bed adsorption (EBA) technology, precipitate protein isolation by membrane electrophoresis (PrIME+), and the affinity cascade (PPPS) all demonstrate progress in different parts or in differen…
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Evolution of the Monoclonal Antibody Purification Platform
The authors discuss the evolution of the purification platform for manufacturing of mAb therapeutics.
Monoclonal antibodies (mAb) increasingly form the majority share of the product pipeline…
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Report from the Eighth International Plasma Product Biotechnology Meeting Melia Salinas, Lanzarote, Spain
Report from the Lanzarote meeting May 13th – 17th, 2013
Yet another well-attended and successful Plasma Product Biotechnology (PPB) meeting has been held. The meeting focu…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
Using this process, the pH of the Protein A affinity chromatography eluate was adjusted upward after pH inactivation and loaded directly onto the anion exchange resin. The anion exchange flow-through …