Search results for " affinity" in Articles / App Notes
Article
Get Started: Purification of mAb Variants - eLearning
The success of monoclonal antibodies (mAbs) as therapeutics and the multi-domain structure of antibodies has made it possible to create antibody variants, such as bispecific antibodies (bsAbs)…
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A deep dive into optimizing AAV capture and polishing to maximize both yield and purity
The affinity capture step maximizes recovery and efficiently removes impurities.
Join this webinar to:
• Learn how to optimize affinity capture
• Discover critical parameters that enhance f…
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Technology Innovations Improve Process Chromatography Performance
Specific affinity resins are also being developed that enable an effective and straightforward capture step that results in high purity. We can see this trend in new biological targets (e.g., adeno-as…
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Addressing the Complex Nature of Downstream Processing with QbD
“For instance, protein A or affinity resins in general, as well as flow through steps, display these properties since these steps in most cases build on a fundamental understanding of the physiochemic…
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Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
What is your response to these concerns?
It is true that Protein A affinity chromatography resins have higher cost per liter compared to, for example, ion exchangers or HIC resins. It is also tru…
Article
Improvements in Protein A Chromatography
Do you expect a similar approach with affinity resins and purification platforms to be used in these cases a swell?
Some molecules, like those having Fab domains, are amendable to the use of pla…
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Protein Purification Troubleshooting Guide
Practical advice to significantly impact your chromatography workflow
Put this poster on your lab wall to quickly identify and resolve protein purification problems when and where they happen. I…
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A Platform Approach to Purification of Antibody Fragments
However, with recent developments of novel affinity chromatography resins, there are new emerging possibilities. Here, we present affinity chromatography resins that offer the possibility of a platfor…
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Resolving a bottleneck in screening and characterization of recombinant antibody fragments using Biacore 4000
This method provides rapid screening in combination with data-rich selection of fragments with high affinity and the desired kinetic behavior. Kinetic analysis at different temperatures provided addit…
Article
Tackling Analytical Method Development for ADCs
Bioanalysis in biological fluids
From an immunoassay perspective, the development and validation of methods for the bioanalysis of ADCs require the consistent availability of high affinity, high s…