Search results for " affinity" in Articles / App Notes
Article
SEC in the Modern Downstream Purification Process
Biomolecules typically should not bind to an SEC resin like they do in ion-exchange chromatography, affinity chromatography, hydrophobic interaction, or mixed-mode chromatography. As a result, mobile-…
Article
Ligand-Binding Assays and the Determination of Biosimilarity
In addition, ligand-binding assays enable the characterization of the potency of different biosimilars and provide crucial information on the affinity (dissociation rate constant, KD) and selectivity …
Article
Optimizing Resin Performance with Disposable Chromatography Solutions
Shawn Knol/GETTY IMAGES
By: Randi Hernandez
BioPharm International, spoke to Fredrik Lundström, product manager, Cytiva; Jonathan Royce, bioprocess senior product manager of antibody affinity …
Article
Defining Critical Quality Attributes for Monoclonal Antibody Therapeutic Products
To study the effects of glycan structure on the clearance of an Fc receptor fusion protein, an affinity extraction method was developed that could specifically recover the drug from cynomolgus monkey …
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Feasibility has been demonstrated for a tandem downstream process for the purification of mAbs employing an affinity Protein A capture step followed by a flow-through anion-exchange (AEX) step and a v…
Article
Quality by design for biotechnology products—part 1
…proved stability; recombinant and chemical fusion approaches for improved half-life and potency; and affinity maturation by phage display or transgenic mouse (e.g., xenomouse, Humax, and velocity mou…
Article
Introducing the VH3 ligand ELISA kit
Accurate testing for ligand leakage from affinity resins is a key part of this process, ensuring compliance with regulatory standards.
Our new VH3 ligand ELISA kit - the latest addition to Cytiva…
Article
Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
Protein L resins bind to kappa light chain and are often used to purify antibody fragments such as dAbs and Fabs — but they can also be used to remove product-related impurities from bispecif…
Article
You are invited to attend DiPIA 2024
What is DiPIA?
DiPIA, or Developments in Protein Interaction Analysis, is a global scientific conference hosted by Cytiva. We bring peers together from across the industry to share best p…
Article
Purification strategies for antibody-derived entities: bispecifics and fragments
The diversity of antibody variants in today’s pipeline presents unique challenges for purification. Purification platforms used for many monoclonal antibodies (mAbs) need to be adopted for ant…