Search results for " affinity" in Articles / App Notes
Article
Antibody Production in Microbial Hosts
Recent developments in aglycosylated antibodies possessing similar antigen-binding affinity and engineered-specific FcγR binding has led to an enormous interest in these products. In this article, the…
Article
Re-use of Protein A Resin: Fouling and Economics
Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography has remained the de facto capture step for purification of monoclonal antibod…
Article
Challenges of Protein Aggregation During Purification
For example, certain CaptureSelect affinity ligands/resins from Thermo Fisher Scientific have been designed to favor elution under less acidic, more neutral pH conditions, according to Gebski.
Ref…
Article
Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
The first method is using nonaffinity chromatography steps (AIEX + heat treatment + HIC), while the second method is using the novel affinity Cytiva™ Protein Select™ resin for the capture step, follow…
Article
Chromatography innovations for the diversified antibody pipeline
Our MabSelect™ VH3 affinity resin interacts with the variable heavy chain of the VH3 sequence family of human antibody, delivering high binding capacity and efficient separation of product-related imp…
Article
Dynamic Binding Capacity in Fiber-Based Chromatography
Fiber chromatography matrices such as Fibro PrismA can bind a large amount of protein in a fraction of the purification time required for an affinity resin. Just like with resins, the dynamic binding …
Article
Selection Guide: Columns and Resins for Antibody Purification and Immunoprecipitation
Download the latest version of the selection guide Columns and Resins for Antibody Purification and Immunoprecipitation to help you select the most suitable affinity column for purifying your antibody…
Article
Process Chromatography Selection for Downstream Processing Applications
By contrast, immobilized metal affinity chromatography is not often used because tagged proteins are uncommon in process scale.
Becerra-Arteaga (MilliporeSigma): Ion-exchange chromatography is mos…
Article
Optimize Column Loading Strategy to Gain Productivity in Protein Purification
Have you ever applied the concept of variable loading to gain productivity? By varying residence time (RT) over the loading phase, productivity from an affinity chromatography step can be significantl…
Article
Trends in Downstream Bioprocessing
It involves the removal of insoluble cell debris and particulates; product isolation through extraction, adsorption, ultrafiltration, or precipitation; product purification through affinity chromatogr…