Search results for "buffers" in Articles / App Notes
Article
Development of Purification for Challenging Fc-Fusion Proteins
Buffers were formulated in an acetate buffer system of pH 4.0 to 5.5 with 0 to 250 mM sodium chloride (NaCl) concentration. The filter plate was shaken between stages at 1200 revolutions per minute (R…
Article
Making the Move to Continuous Chromatography
The manufacturer just needs the right equipment and expertise; the processes use the same buffers and resins as used in their current batch processes,” he says. “Ultimately, if you want to work faster…
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Fluid Handling in Biopharma Facilities
BioPharm: Which fluids represent the largest volumes within a facility?
Blomberg (Meissner): Typically within a biopharmaceutical manufacturing process that employs single-use systems, buffers and …
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Other advantages of developing a method using a quaternary solvent system includes ease of mobile phase composition control, which can greatly reduce the labor needed to make and change a variety of b…
Article
QbD and PAT in Upstream and Downstream Processing
Control of these critical buffers is managed through batch preparation and release prior to use based on acceptable pH and conductivity ranges. Process capability is sufficient to reproducibly prepare…
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Single-Use for Downstream Chromatography: Benefit or Hindrance?
Prefilled BPCs have only made the single-use option more popular, he says, noting that BPCs prefilled with water for injection or purification buffers can be delivered to customers, eliminating the ne…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
Newer classes of biotherapies will require innovations in processing technology.
By Glen R. Bolton, Bernard N. Violand, Richard S. Wright, Shujun Sun, Khurram M. Sunasara, Kathleen Watson, Johna…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
A major advantage of SPR-based analysis is its ability to estimate the association and dissociation rate constants, an …
Article
The Challenge of Disruptive Technologies in Bioprocessing
“We see end-users transitioning away from internal manufacturing of downstream processing buffers and focusing on core activities; they are looking to outsource these non-core activities to ease the b…
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Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
The objective of this study was to assess the impact of manufacturing-scale, freeze-thaw conditions on aggregation and subvisible particle formation of a monoclonal antibody solution (mAb-A; IgG1) u…