Search results for " separation" in Articles / App Notes
Article
Technology Innovations Improve Process Chromatography Performance
By Feliza Mirasol
Selecting an appropriate separation technology for biologic manufacturing is a crucial task in maximizing productivity in downstream bioprocessing. Biomanufacturers consider se…
Article
Recent Advances in the Use of Exoglycosidases to Improve Structural Profiling of N-glycans from Biologic Drugs
Fluorescently labeled N-glycans are commonly analyzed by separation using high- or ultra-high-performance liquid chromatography (HPLC or UHPLC, respectively) coupled to fluorescence detection (FLR), o…
Article
Ensuring Viral Safety of Viral Vaccines and Vectors
Chromatographic separation of empty versus full capsids with a similar charge remain a challenge. Density separation using ultracentrifugation is possible but is challenging at GMP manufacturing scale…
Article
Preclinical Evaluation of Product Related Impurities and Variants
First, a rigorous method for separation of these variants is needed (23) and is non-trivial due to the fact that the physicochemical properties of these variants are nearly identical. Researchers have…
Article
Downstream Processing for Cell-Based Therapies
While highly specific labeling separation systems may be suitable within low volume production (proof-of-principle, small-scale studies, or autologous applications), it is extremely unlikely these wil…
Article
Host-Cell Protein Measurement and Control
Supplementary to HCP ELISA, traditional HCP separation and visualization methods, such as 1D and 2D sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), remain useful tools for HCP ch…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
For purification of basic and neutral proteins, the CHA column is usually equilibrated with phosphate buffer at a neutral pH to utilize a cation exchange mechanism for the separation of protein monome…
Article
Chromatography for the Diversified Antibody Pipeline
It may place more work on the main polishing steps, however, MabSelect may interact with the classes of DH3 in the antibodies—which enables separations targeting the constant DC region. We can see the…
Article
Gene Therapies Push Viral Vector Production
Gene Therapies Push Viral Vector Production
Viral vectors show promise as a delivery mechanism for gene therapy, but which virus types are commercially viable?
By Feliza Miraso…
Article
Going Small to Achieve Success on the Commercial Scale
Conducting the required number of studies on the commercial scale is not practical; scale-down models are therefore employed to determine optimum conditions for downstream separation processes, includ…