Search results for " expression"
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Addressing the Challenges in Downstream Processing Today and Tomorrow
In addition to high cell density mammalian expression, expanded utilization of other expression systems such as microbial and yeast will support these newer biotherapeutics (BioTx). The diversity of B…
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Milestones and Moderate Progress in 2012 Drug Approvals
These figures once again reflect the ongoing dominance of mammalian-based expression systems in terms of biopharmaceutical manufacture.
Digging a little deeper reveals that the majority …
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Gene Therapies Push Viral Vector Production
The commercial viability of other virus types also continues to be explored, particularly for treatments that require a larger payload, transient expression, or insertion of a gene of interest in the …
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Single-Domain Antibodies for Brain Targeting
Bacteria are favored for expression of V-like domains, while human sdAbs can be expressed at high yields (> 50 mg L-1 in Escherichia coli and > 0.5 g L-1 in Pichia pastoris shaker flask cultures) (4).…
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Bioprocessing Advances in Vaccine Manufacture
These advances include:
•Single-use systems (SUS)/disposable bioprocessing systems
•Modular/transportable bioprocessing facilities
•Novel expression systems/improved cell lines
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Elucidating Biosimilars Characterization
…on of similarity is complicated by nature of the fact that biologic products are expressed in living expression systems and differences regarding the manufacturing processes, be it cell lines used, m…
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Impact of Media Components on CQAs of Monoclonal Antibodies
Major mammalian expression systems for the production of biotherapeutics include NS0 cells, baby hamster kidney (BHK) cells, hybridoma cells, PER.C6 cells, human embryonic kidney (HEK) cells, and Chin…
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Using Quality by Design to Develop Robust Chromatographic Methods
In other industries, the understanding of data “quality” is commonly communicated through an expression of the “uncertainty” associated with a measurement “result”. This uncertainty is treated with eq…
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Evolution of the Monoclonal Antibody Purification Platform
Table I summarizes some of the process development challenges with mAb quality from the various expression systems and the tools and unit operations options for addressing these challenges.
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Fusion Proteins Pose Manufacturability Challenges
“These fusion proteins include an Fc region, are taken from an IgG, that extends the serum half-life of the fusion partner, facilitates expression, and allows purification by protein A affinity chroma…