Search results for "expression" in Articles / App Notes
Article
Platform Approach Speeds Process Development
An expression platform has demonstrated fast-track development of high-quality, high-titer processes for producing biopharmaceuticals from CHO cells. The platform includes vector design, cell line eng…
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Genetic Vaccine Platforms Demonstrate Their Potential
“Viral proteins are developed and manufactured in cell-based expression systems, and they require time to generate a clonal cell line expressing the protein-of-interest and then optimize the manufactu…
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Reimagining Affordable Biosimilars
Cell-based binding in SPR and BLI takes into account the cell-surface expression of receptors, allowing label-free detection, binding, and saturation events. Recent advances in protein interaction stu…
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State of the Biopharmaceutical Industry: Lessons from 2018 Survey Data
I also continue to be surprised that industry continues to ignore the benefits of adopting new and improved expression systems. Back in 2010, BioPlan published the first and still only directory of co…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
The array included 84 genes for protein expression, folding, and secretion. Gene level changes from day 9 to day 11 in the protein-production phase were investigated in this assay, using two samples p…
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Trends in BioPharma Approvals in 2013
In terms of expression systems used, a total of eight of the products approved are expressed in mammalian cell lines (seven in CHO cells, one in Sp20), six are produced in yeast-based systems, four in…
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Regulation of Biosimilars: A Matter of Variability, Similarity, and Comparability
Another potential source of unacceptable incompatibilities is the use of contrasting expression systems in biosimilar proteins. Windisch pointed out that a distinction had to be made between different…
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Virus-like Particles as Therapeutic Moieties of the Future
At least 110 VLPs have been constructed so far from 30 different virus families (1), and a variety of expression systems ranging from bacteria to mammalian cell systems have been used for their produc…
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Can Vaccine Development Be Safely Accelerated?
The platform technology, which does not require prior knowledge of a protein’s quaternary structure, therefore facilitates the expression of recombinant viral glycoproteins without loss of native anti…
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Improving Upstream Predictability
Some of the most significant gains have been made in cell line expression, advanced sensors, and automation, says Atul Mohindra, R&D director for biomanufacturing at Lonza Pharma and Biotech. “Using a…