Search results for " fragment" in Articles / App Notes
Article
Re-use of Protein A Resin: Fouling and Economics
Proteases in harvested cell culture fluid are known to be responsible for proteolytic cleavage of Protein A ligand into fragments ranging in molecular weight from six to 40 kDa (27). Protein A ligand …
Article
Fermentation for the Future
Newer drug substances, such as single-domain antibodies, peptibodies, or antibody fragments that contain the effective domains, have recently been produced using microbes.
Microbial expression is …
Article
Optimizing Resin Performance with Disposable Chromatography Solutions
…ns based on Protein L) for the purification of a next-generation of biotherapeutics such as antibody fragments.
Tingley (Repligen): Yes, the single-use chromatography products we are discussing w…
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
They also often do not consist of discreet populations, such as monomers and low oligomers or fragments. “Real proteins may be partially or completely disordered, contain significant hydrophobic resid…
Article
Novel Vaccine Technologies Meet the Need for Pandemic and Therapeutic Solutions
George adds that the incorporation of a xenotypic antibody fragment makes the entire molecule “foreign” and thus more immunogenic. Production of the vaccines in insect cells is also beneficial, becaus…
Article
EMA Collaborates with HTA Assessment Networks
Despite Europe being a pioneer in the development and commercialization of biosimilars, it has a fragmented market for the products with national health authorities following different policies for th…
Article
Early Communication with Regulators is Essential for SMEs
A major objective is the elimination of fragmentation in the fields of scientific research and innovation. Among the EU’s regulatory agencies in pharmaceuticals, the most active in championing innovat…
Article
A Platform Approach for the Purification of Domain Antibodies (Dabs)
This Application note describes a three-step purification process of a domain antibody (Dab) expressed in the periplasm of E. coli. First, a capture step using Capto™ L was used to reduce E. coli host…
Article
Elucidating Biosimilars Characterization
…mbined use of
collisional-induced dissociation (CID) and/or electron-transfer dissociation (ETD) fragmentation strategies are employed. As mentioned previously, LC, MS, and LC-MS approaches are w…
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The bead core contains octylamine ligands that bind a broad range of substances including proteins, peptides, and nucleotide fragments. The multimodal function of the chromatography medium, utilizing …