Search results for "buffer"
Article
Implementing Inline Conditioning to Advance Process Intensification
With inline conditioning buffers can be prepared inline from buffer concentrates or stock solutions, and directly integrated with the chromatography or filtration step. This eliminates the need for in…
Article
Modeling the Degradation of mAb Therapeutics
Environmental factors that can have an impact on the protein include pH, temperature, salt concentration, buffer type, protein concentration, ionic strength, mixing, shear, metal ions, pressure, freez…
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Improving Process-Scale Chromatography
Capacity in a capture step such as Protein A is critical in reducing cost and increasing the ease of use through reductions in column size, buffer usage, and overall footprint of the unit operation,” …
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What’s New in Upstream Technologies
Wet, one-point standardization before startup allows for the sensor’s characteristics and functionality to be verified before any solutions or buffers are added to the bioreactor bag. The standardizat…
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Development of Purification for Challenging Fc-Fusion Proteins
A low pH buffer is routinely used to elute the mAb product, which is followed by a viral inactivation step.
Anion exchange chromatography (AEX) (6-8, 10-11), typically serves as a polishi…
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Making the Move to Continuous Chromatography
Buffer consumption is also reduced.
Manufacturers are always trying to optimize their processes, particularly as product titers increase, according to Levison. In the case of antibodies, the in…
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Fluid Handling in Biopharma Facilities
BioPharm: Which fluids represent the largest volumes within a facility?
Blomberg (Meissner): Typically within a biopharmaceutical manufacturing process that employs single-use systems, buffers and …
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Samples for iCIEF also must undergo additional sample preparation steps, frequently including a buffer exchange before analysis.
Ion Exchange Chromatography
Ion-exchange chromatography (IEC)…
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QbD and PAT in Upstream and Downstream Processing
Control of these critical buffers is managed through batch preparation and release prior to use based on acceptable pH and conductivity ranges. Process capability is sufficient to reproducibly prepare…
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Single-Use for Downstream Chromatography: Benefit or Hindrance?
Another area where single-use is gaining ground downstream is in buffer preparation, storage, and dispense. “Single-use mixers, coupled with single-use bioprocess containers (BPC), can increase a DSP …