Search results for " ligand" in Articles / App Notes
Article
Comparing Protein A Resins for Monoclonal Antibody Purification
Protein A is the affinity chromatography ligand of choice for the first antibody capture step, because its high selectivity gives excellent purity (typically > 99%) and high yields. Furthermore, Prote…
Article
Ligand-Binding Assays and the Determination of Biosimilarity
Consequently, functional assays, such as ligand-binding assays (LBAs) and mechanism of action (MOA) cell-based assays (CBAs), must be developed to effectively assess biosimilars. In many cases, LBAs a…
Article
Technology Innovations Improve Process Chromatography Performance
Sexton notes that ligand development has helped enhance the robustness of processes in development. “A ligand that has a higher affinity for its substrate or that is more resistant to harsher cleaning…
Article
Next Generation Process Chromatography
Binding capacities, range of selectivity, and the stability of affinity ligands have been vastly improved. One of the main remaining challenges is that each purification cycle today typically takes >3…
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
The enzyme-linked immunosorbent assay (ELISA) is often used to assess ligand binding; however, it is an indirect method and the output is limited to end-point data. By contrast, label-free technologie…
Article
SEC in the Modern Downstream Purification Process
…matographic modes that are driven by chemical interactions between the sample being purified and the ligand conjugated to the stationary phase.
New applications for SEC
SEC has experienced a …
Article
Evolution of the Monoclonal Antibody Purification Platform
Use of multimodal chromatography
Recently, mixed-mode, ion-exchange ligands with enhanced binding strength for aggregates through the addition of hydrophobic functionality have been brought to mar…
Article
Chromatography for the Diversified Antibody Pipeline
We can see the protein and understand the necessity for having other tools targeting regions on the antibodies such as Kappa ligand or the Lambda ligand. These were developed for fragments and not alk…
Article
Process Chromatography Selection for Downstream Processing Applications
Levison (Pall): With the introduction of continuous chromatography and optimization of flow through approaches to membrane chromatography, novel ligands, and base matrices may gain broader application…
Article
Top Tips for Successful Development of Antibody Chromatography Processes
We have a large array of different ligands. So to understand these, what are the benefits? What are their kind of weak spots? So look at different capacities, flow rates, efficiency, etc. There's alwa…