Search results for " columns"
Article
Downstream Processing Continues to Worry CMOs
(Figure courtesy of author)
Chromatography and ultrafiltration
When it comes to specific steps that contribute to downstream-related capacity constraints, chromatography columns continue to be…
Article
Using Quality by Design to Develop Robust Chromatographic Methods
Quality-by-design principles can be used to understand chromatographic measurement system variability.
Sep 2, 2014
By: Melissa Hanna-Brown, Kimber Barnett, Brent Harrington, Tim Graul, Jim…
Article
Trends in Downstream Bioprocessing
• We need better affinity columns (protein A).
• The problem is buffer capacity and need for in-line dilution.
Which Purification Steps Are the Main Culprits?
Not all purification steps…
Article
Single-Use for Downstream Chromatography: Benefit or Hindrance?
Hindrance or help?
Many disposable chromatography columns meet cGMP and industry standards, and also offer reproducibility, scalability, speed, ease-of-use, and operational safety.
However, chr…
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The possibility to serially connect Capto Q and Capto Core 700 media columns allows for enhanced system utilization and a reduced overall process time.
Before the sterile filtration step, the vi…
Article
Insider Recommendations for Getting Started with Mechanistic Modeling of Chromatography
Of course, Cytiva does also provide tools to help in that regard, such as precalibrated columns. But unlike a statistical model, really it's having that singular correct measure of the systems is the …
Article
Technology Innovations Improve Process Chromatography Performance
“Traditional, resin-based chromatography columns are often oversized due to throughput limitations and are ill-suited to flexible manufacturing. These factors impose challenges on the design of purifi…
Article
Multidimensional Scale-Up of a Monoclonal Antibody Capture Step, Using ÄKTA™ pilot 600
Fast and efficient process development and scale-up contributes to a shortened time to market. In this work, a multidimensional scale-up (change of both column diameter and bed height) of a mAb capt…
Article
Comparing Protein A Resins for Monoclonal Antibody Purification
A prototype Protein A resin is evaluated for purification performance, reusability, and cost performance.
With greater economic pressure on monoclonal antibody (mAb) production for therapeutic …
Article
Instructional Videos for Bioprocess Equipment
Modules also cover column packing and maintenance for pilot and process-scale columns.
Each mediabook contains detailed videos for the equipment and the software interface.
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