Search results for " columns"
Article
Continuous Chromatography: Trends, Definitions and Approaches
All of the acronyms describe the same thing: a chromatography capture step-based utilizing multiple columns operated in a merry-go-round manner, where columns moved against the direction of feed flow.…
Article
Development of Purification for Challenging Fc-Fusion Proteins
HIC and CEX columns were packed in a vantage 0.66-cm or above column (MilliporeSigma) with a bed-height of 10 to 20 cm and residence times of five to six minutes were maintained upon scale-up.
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Article
Unifying Continuous Biomanufacturing Operations
There is difficulty associated with sterilizing chromatography columns; there are “no sterility testing [methods] for chromatography columns,” according to the study authors (3). Also, Klutz and team …
Article
Continuous Manufacturing: A Changing Processing Paradigm
They also note that continuous capture typically requires sterile chromatography columns. “For downstream polishing steps, the infancy of multi-column chromatography systems is a limitation,” they add…
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
The combination of SEC with downstream light scattering analysis and the development of columns that can be used for ultra high-pressure liquid chromatography (UHPLC)-based SEC are overcoming many of …
Article
Evaluating the Use of Continuous Chromatography
…ation step is connected to an upstream perfusion or fed-batch bioreactor, and, by having two or more columns in the loading zone, you can overload the first column [because] any product/target molecu…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
…and elution conditions, can be explored in a shorter time frame compared with when using small-scale columns, which are operated in a sequential mode. In this study, a robotic liquid handling system …
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
If used correctly, these new analytical methods can reduce analysis and product development time.
ABSTRACT
Monoclonal antibodies represent a significant portion of sales in the biopharma…
Article
Reducing Cross-Contamination Risks in Process Chromatography
Preparatory and post-processing steps such as column packing, validation of packed-bed efficiency, verification of endotoxin clearance, unpacking, cleaning of unpacked columns, etc., can also be elimi…
Article
Improving Process-Scale Chromatography
…e chromatography steps are connected in series with in-line adjustment of process conditions between columns to ensure optimized loading conditions during the next step, is being evaluated at large s…