Search results for " protein" in Articles / App Notes
Article
Industry Adoption of Single-Use Systems Remains Low
…facility and conducts development and GMP manufacture of monoclonal antibodies and other recombinant proteins at its San Diego facility.
Under their agreement, Sartorius Stedim will equi…
Article
Roadmap Leads to Innovative Biomanufacturing Strategies
The initial emphasis of the Biomanufacturing Technology Roadmap has been on the manufacture of recombinant proteins and, even more specifically, on monoclonal antibodies (mAbs).
With successive …
Article
Multidimensional Scale-Up of a Monoclonal Antibody Capture Step, Using ÄKTA™ pilot 600
The process, performed with MabSelect™ PrismA Protein A chromatography resin, was scaled up 20-fold from a 70 mL HiScale™ 26 column to a 1400 mL AxiChrom™ 100 column. Equivalent mAb recovery and purit…
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
This work describes the capabilities of ÄKTA readyflux when used in ultrafiltration/diafiltration (UF/DF) applications. The process was conducted in an automated manner based on methods created …
Article
Tips for Purification Method Editing: Learnings from the Webinar
During the ÄKTA club webinar, “Tips for Protein Purification Method Editing in UNICORN software,” the basics of the UNICORN 7 software were presented. Useful tips and features were demonstrated, …
Article
Evaluation of HEK293 Cell Growth and Adenovirus Productivity in HyClone™ CDM4HEK293 Medium
Human embryonic kidney 293 (HEK293) cells are widely used in production of recombinant viruses and proteins because of their stable growth, high transfection level, and ability to produce high viral t…
Article
Patenting Prospects for Cell-Based Therapies
The history of biomedical advances over the past 50 years has focused predominantly of increasing complexity, from small-molecule therapeutics to antibodies, cytokines, blood-clotting factors, and pro…
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Current Innovations and Practices To Address Microbial Contamination in Downstream Bioprocessing
Bioburden control is an area of serious concern for anyone making mAbs or other biologicals. Manufacturers’ efforts to avoid microbial contamination are today being complemented by development…
Article
How to Address Bioburden Challenges: Insights and Tips
Another important area where suppliers can contribute is sanitization and storage procedures for protein A chromatography resins, which are widely used to purify mAbs.
Learn more about insights an…
Article
The New World of Biopharmaceutical Manufacturing
Today, we can express proteins in cell culture, so the approach to downstream processing is completely different. In the past five years, the biggest advances have been in the introduction, developmen…