Search results for " HEK293" in Articles / App Notes
Article
Evaluation of HEK293 Cell Growth and Adenovirus Productivity in HyClone™ CDM4HEK293 Medium
This application note demonstrates growth of HEK293 cells in CDM4HEK293 medium in both shake flask and bioreactor cultures. Adenovirus productivity was evaluated in shake flask cultures. Performance i…
Article
Evaluation of Hek293 Cell Growth and Adenovirus Productivity in HyClone CDM4HEK293 Medium
This application note demonstrates growth and adenovirus productivity of HEK293 cells and explores the key factors involved in the optimization of cell culture performance and scalability.
Cli…
Article
rAAV Production Using HEK293 Cell Line and Transfection Medium
Improving the performance of a scalable AAV process in Xcellerex™ XDR-10 and XDR-200 bioreactor using HyClone™ peak expression medium.
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Article
Single-Use Technologies Prove Effective for Viral Vector Process Development
New media formulations have been developed that allow direct adaptation of HEK293 adherent cell lines to serum-free suspension growth conditions, according to Sanderson. Suspension-adapted cell lines …
Article
Recombinant Adeno-Associated Virus Type 5 Production Process
A scalable recombinant adeno-associated virus (rAAV) type 5 production process was developed using transfected HEK293 suspension cells.
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Article
Cell culture process development for AAV vector production in suspension cells
Adaptation of HEK293 suspension cell line for transient transfection and scalable adeno-associated virus (AAV) production.
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Commercial Production of Gene Therapies Using Suspension or Adherent Cell Lines
Mammalians cells, especially HEK293, are popular hosts; however, insect cells are also used. These originate as tissue and thus favor growing in adherence but are also being adapted to grow in suspens…
Article
Production of Recombinant Adeno-associated Virus Vectors Using Microcarrier Culture of HEK293T Cells
As of March 2020, more than 4310 gene therapy clinical trials have been performed worldwide, with only 518 of them in Phase III and Phase IV. As the gene therapy projects enter clinical Phase III or g…
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Biopharmaceutical Process Development: 2018 Lessons and 2019 Predictions
…escribing the development of downstream chromatography steps for the purification of adenovirus from HEK293 cell culture harvest.
Single-Use Technologies Are Still Growing—But Not Without Challen…
Article
Developing an Efficient Adenovirus Downstream Purification Process
This application note describes the development of downstream chromatography steps for purification of adenovirus from HEK293 cell culture harvest.
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