Search results for "antibody" in Articles / App Notes
Article
Process Chromatography: Continuous Optimization
In fact, according to Parra, highly selective affinity resins with antibody-like specificity can significantly improve a purification process making it simpler and more economical for a variety of mol…
Article
Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
Nesta
Abstract
The objective of this study was to assess the impact of manufacturing-scale, freeze-thaw conditions on aggregation and subvisible particle formation of a monoclonal antibody sol…
Article
Elucidating Biosimilars Characterization
Compare this compound with the monoclonal antibody trastuzumab, which has a reported chemical formula of C6470H10012N1726O2013S42 and a molecular weight of 145531.50 g/mol, approximately 260 times the…
Article
Best Practices for Selecting a Top-Quality Cell Line
“A balanced plasmid ratio usually has dramatic impact on some formats of bispecific antibody cell line development to minimize impurities,” she explains. Factors such as cell growth and metabolism, as…
Article
Improving Process-Scale Chromatography
The development of new resin chemistries including enhancements to Protein A for monoclonal antibody (mAb) capture, increasing use of automation, implementation of single-use technologies, and explora…
Article
Analysis of Glycosylation in Biosimilars
This compares the MS traces of the N-glycans released from two different glycoproteins: a classic antibody and a secretory glycoprotein. Antibodies produce glycans that are relatively small and simple…
Article
Implications of Cell Culture Conditions on Protein Glycosylation
Figure 1: Graphic showing IgG antibody structure, sites for glycosylation, and potential variability.
Proteins are delicate molecules compared with small-molecule drugs and present multiple stabili…
Article
Ligand-Binding Assays and the Determination of Biosimilarity
…lecule are involved in binding with different activities (the Fab and Fc regions, which bind to the antibody target/ligand and Fc receptors [CD64, CD32, CD16, and FcRN], respectively). While in-vitro…
Article
Trends in BioPharma Approvals in 2013
Engineered antibodies
Genentech’s Gazyva represents another milestone in antibody approvals, being the first glycoengineered mAb to come on the market in the US. mAbs are glycoproteins, carrying a…
Article
ADC Development Robust Despite Lackluster Performance
The ADC construct combines a targeted monoclonal antibody (mAb) with a cytotoxic agent, linked together with a stable linker technology. An ADC enables the specific delivery of chemotherapeutics to tu…