Search results for "bioreactors"
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
First, the availability of high-throughput miniature bioreactors has made it possible to evaluate many more conditions. This has enabled design of experiment (DoE) approaches, such as response surface…
Article
Best Practices in Qualification of Single-Use Systems
Aseptic connector devices, mixer bags, storage bags, and bioreactors followed. Currently, single-use systems (SUS) also include disconnectors, disposable filling needles, and sensors. The technology i…
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Evaluating the Use of Continuous Chromatography
…t or sometimes over 24 hours, the larger volumes processed continuously from perfusion or fed-batch bioreactors must be processed over several weeks or up to a month. [This] can make it more difficul…
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Simplify-bioreactor scale up and scale down: applications and tools
Scaling the physical environment in a bioreactor is a frequent task in each product development project. A well-designed scaling strategy, workflows, and easy-to-use tools will facil…
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Best Practices for Selecting a Top-Quality Cell Line
Before scaling up to bioreactors, the productivity level for monoclonal antibodies (mAbs) should be 2–7 g/L (fed-batch culture in shake flask) or greater, observes Selexis CEO Igor Fisch. Once scaled …
Article
Ensuring Viral Safety of Viral Vaccines and Vectors
Extraneous vesiviruses, however, have recently appeared in bioreactors (2–5), and porcine circovirus type 1 (PCV-1) contamination of oral rotavirus vaccines was first reported by a metagenomics analys…
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Optimizing Late-Stage and Commercial Cell-Culture Processes
He also anticipates seeing more complex processes and smaller, single-use technology bioreactors for production. “Accelerated clinical development becomes more common and faster, leading to very short…
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Challenges in Cell Harvesting Prompt Enhancements
Biomanufacturers have been shifting from large stainless-steel bioreactors in the thousands-of-liters range to smaller, disposable bioreactors (2).
The need to address this bottlenecking issue …
Article
Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
For example, one can consider 2 x 2000-L bioreactors running a typical 14-day incubation period staggered a week apart, which equates to one batch produced each week. A typical batch at 2 g/L with a 7…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
…ization, many of these studies are performed using cells grown in shake flasks or small bench-scale bioreactors under nonoptimized cell culture conditions. Therefore, it is crucial to further evaluat…