Search results for "Recovery" in Articles / App Notes
Article
Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
This strategy, initially applied to the recovery of cell sheets from t-flask type culture devices (5), has been extended successfully to microcarrier-based cell cultures (3). A key issue in this appro…
Article
Going Small to Achieve Success on the Commercial Scale
“Demonstration of both individual unit and linked unit output (recovery, product quality attributes, product purity, chromatographic profiles/transition analysis, etc.) comparability between small and…
Article
Selecting the Right Viral Clearance Technology
…e a purified protein that meets specifications and are developed to maximize purity with reasonable recovery. Only then is a chromatography step tested for viral clearance. “One solution is to add a …
Article
Automated harvesting and 2-step purification of unclarified mammalian cell-culture broths containing antibodies
The mean recovery of purified material from the culture-broth was 66.7%, representing a 20% increase compared to that of the manual process. Moreover, the automated process reduced by 3-fold the amoun…
Article
Risk Assessment and Mitigation in Biopharmaceutical Manufacturing
It is then a matter of developing recovery plans in the event of a disruption.
References
1. ICH, Q9 Quality Risk Management (ICH, June 2006).
2. ICH, Q10 Pharmaceutical Quality System (IC…
Article
Re-use of Protein A Resin: Fouling and Economics
It has been shown that the extended use of a resin results in decrease in its binding capacity, a decrease in product recovery, and a loss of Protein A ligands (9). Recent innovations in Protein A-aff…
Article
Challenges of Protein Aggregation During Purification
Changing the surface chemistry
Downstream processing for recovery and purification is achieved in several steps by exploiting the differences in the affinities, charges, sizes, hydrophobicities, a…
Article
Q&As with Industry Leaders
…partment of Biotechnology, BOKU
Recovery of Biological Products Conference Series
Günter Jagschies, Cyt…
Article
Trends in Downstream Bioprocessing
Downstream processing involves the recovery and purification of protein therapeutics for biopharmaceuticals, vaccines, and other biologics. It involves the removal of insoluble cell debris and parti…
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Samples containing antibodies in cell culture fluid were injected onto a Protein A column for antibody recovery, then the purified antibody sample was automatically injected onto the size exclusion co…