Search results for "Recovery" in Articles / App Notes
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
Protein recovery was ≥ 99%, after 5‑times concentration and 5‑fold diafiltration. The process was performed according to set specifications for a tangential flow filtration (TFF) process, with the…
Article
Process Chromatography: Continuous Optimization
The result, according to Westman, is high-purity recovery under generic conditions in just one separation step and enables a plug-and-play approach that leads to reduced process development times. In …
Article
The New World of Biopharmaceutical Manufacturing
They will also be integrated into recovery/purification and be operated in semi-continuous or continuous mode. The number of drugs that are produced by non-mammalian expression systems, such as yeast …
Article
Managing Residual Impurities During Downstream Processing
Requirements for system suitability (negative control solution, sensitivity, and linearity), spike recovery of extracted samples, and precision of sample replicates are covered but the limit for resid…
Article
Mechanistic Modeling of Preparative Ion-Exchange Chromatography
For example, for the commonly used ion-exchange chromatography, pH, ion concentration, gradient, and stationary phase can affect the recovery step as well as the resulting product quality.
For com…
Article
Single-use Bioreactors Have Reached the Big Time
Sartorius has also introduced a new single-use centrifugation system that allows product recovery from the material removed during the cell bleed step. Currently, this product is typically discarded w…
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Efficient purification of meningococcal polysaccharides in a two-step chromatography workflow
This application note demonstrates the purification of meningococcal capsular polysaccharides using chromatography. The described process constitutes a viable alternative to the traditional proce…
Article
Efficient Purification of Pneumococcal Polysaccharides in a Chromatography Workflow
… pneumococcal polysaccharides to eliminate the need for phenol extraction, while increasing process recovery and yield.
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Article
Affinity Chromatography Handbook in 3 Volumes
Affinity chromatography (AC) separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. The techni…
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Affinity chromatography Handbook - Vol. 3: Specific Groups of Biomolecules
Affinity chromatography (AC) separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. The techni…