Search results for " media"
Article
Preclinical Evaluation of Product Related Impurities and Variants
This may be attributed to the complexities related to the use of cell lines, media formulation, and other bioprocessing steps that are prone to variability to varying degrees (4).
The inherent …
Article
Process Development: What May Lie Ahead in 2018?
…n Inspection
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Bioburden Control in the Biopharmaceutical Industry
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Impact of Media Components on CQAs of Monoclonal Antibodies
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Start with the End in Mind GMP Tech Tr…
Article
Development of Purification for Challenging Fc-Fusion Proteins
The main parameters to consider when selecting HIC media and conditions are ligand hydrophobicity, salt type, and salt concentration. The feed stream was sourced from the first polishing chromatograph…
Article
Evaluation of HEK293 Cell Growth and Adenovirus Productivity in HyClone™ CDM4HEK293 Medium
Human embryonic kidney 293 (HEK293) cells are widely used in production of recombinant viruses and proteins because of their stable growth, high transfection level, and ability to produce high viral t…
Article
Process Chromatography: Continuous Optimization
Even so, there are many impurities present in the harvest cell-culture fluid obtained after removal of the cells or yeast and media. Host-cell proteins, host-cell DNA, other process-related contaminan…
Article
N-Glycan Analysis of Biotherapeutic Proteins
The presence of high-mannose glycans results in increased clearance mediated by mannose receptors (14), and as such, when ADCC constitutes the mechanism of action, the presence of high-mannose-type N-…
Poster
Adenovirus production in a single-use stirring tank bioreactor system
By evaluating different cell culture media (CCM) and optimizing the virus propagation in small scale, the process could be successfully established in Xcellerex™ XDR-10 Bioreactor (10 L working volume…
Article
Downstream Processing for Cell-Based Therapies
…carriers, culture surfaces, colonies, or embryoid bodies), these are generally quenched with culture media to preserve cell health by preventing the enzyme from further digesting cell components. Mor…
Article
Speed and Flexibility Are Dual Goals for Biopharma Operations
What new technologies and products are impacting bioprocessing operations?
Recent product introductions for bioprocessing operations—such as advances in bioreactor technology, media, buffers, and …
Poster
Development of a chemically defined medium for virus vaccine production in a duck suspension cell line
A project was initiated to improve the production process for viral vaccines through the use of the EB66® cell line derived from duck embryonic stem cells and proprietary to Valneva. The primary goa…